Salt-assisted LLE combined with field-amplified sample stacking in CE for improved determination of beta blocker drugs in human urine.

Bioanalysis 2014 Feb;6(3):319-34

Biomedical Analysis Lab, Liver & Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Background: A simple and sensitive CE method was developed and validated for the analysis of some beta blockers in human urine.

Methods: In this study, salting-out assisted LLE combined with field-amplified sample stacking method was employed for biological sample clean-up and sensitivity enhancement in CE.

Results: Under the optimal conditions good linearity (r(2) ≥0.998) was obtained, within 0.025-1 µg/ml for propranolol and metoprolol, and within 0.05-1 µg/ml for carvedilol in urine samples. LODs and LLOQs ranged from 0.005 to 0.015 µg/ml, and from 0.025 to 0.05 µg/ml, respectively. The RSDs of intra- and inter-day analysis of examined compounds were less than 4.0%. The recoveries were in the range of 98-119%.

Conclusion: The validated method is successfully applied to determine propranolol, metoprolol and carvedilol in human urine samples obtained from the patients who received these drugs.

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Source
http://www.future-science.com/doi/10.4155/bio.13.303
Publisher Site
http://dx.doi.org/10.4155/bio.13.303DOI Listing
February 2014
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