Cellular transcription factor YY1 mediates the varicella-zoster virus (VZV) IE62 transcriptional activation.

Virology 2014 Jan 12;449:244-53. Epub 2013 Dec 12.

Department of Microbiology and Immunology and the Witebsky Center for Microbial Pathogenesis and Immunology, University at Buffalo, Buffalo, NY, United States.

Several cellular transcription factors have been shown to be involved in IE62-mediated activation. The YY1 cellular transcription factor has activating and repressive effects on gene transcription. Analysis of the VZV genome revealed 19 postulated YY1 binding sites located within putative promoters of 16 VZV genes. Electrophoretic mobility shift assays (EMSA) confirmed the binding of YY1 to ORF10, ORF28/29 and gI promoters and the mutation of these binding sites inhibited YY1 binding and the promoter activation by IE62 alone or following VZV infection. Mutation of the ORF28/29 YY1 site in the VZV genome displayed insignificant influence on virus growth in melanoma cells; but it inhibited the virus replication significantly at day 5 and 6 post infection in HELF cells. This work suggests a novel role for the cellular factor YY1 in VZV replication through the mediation of IE62 activation of viral gene expression.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.virol.2013.11.029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901949PMC
January 2014

Publication Analysis

Top Keywords

cellular transcription
12
vzv genome
8
yy1 binding
8
factor yy1
8
binding sites
8
transcription factor
8
yy1
7
vzv
6
influence virus
4
mobility shift
4
work suggests
4
electrophoretic mobility
4
cellular
4
emsa confirmed
4
virus growth
4
assays emsa
4
cells work
4
shift assays
4
replication day
4
growth melanoma
4

Similar Publications

Differential effects of Sp cellular transcription factors on viral promoter activation by varicella-zoster virus (VZV) IE62 protein.

Virology 2015 Nov 23;485:47-57. Epub 2015 Jul 23.

Departments of Pediatrics and Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA, United States.

The immediate early (IE) 62 protein is the major varicella-zoster virus (VZV) regulatory factor. Analysis of the VZV genome revealed 40 predicted GC-rich boxes within 36 promoters. We examined effects of ectopic expression of Sp1-Sp4 on IE62- mediated transactivation of three viral promoters. Read More

View Article and Full-Text PDF
November 2015

Regulation of the varicella-zoster virus ORF3 promoter by cellular and viral factors.

Virology 2013 Jun 21;440(2):171-81. Epub 2013 Mar 21.

Departments of Pediatrics and Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA 94305, United States.

The varicella zoster virus (VZV) immediate early 62 protein (IE62) activates most if not all identified promoters of VZV genes and also some minimum model promoters that contain only a TATA box element. Analysis of the DNA elements that function in IE62 activation of the VZV ORF3 promoter revealed that the 100 nucleotides before the translation start site of the ORF3 gene contains the promoter elements. This promoter lacks any functional TATA box element. Read More

View Article and Full-Text PDF
June 2013

Analysis of IE62 mutations found in Varicella-Zoster virus vaccine strains for transactivation activity.

J Microbiol 2018 Jun 1;56(6):441-448. Epub 2018 Jun 1.

Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Samsung Medical Center, Suwon, 16419, Republic of Korea.

Live attenuated vaccine strains have been developed for Varicella-Zoster virus (VZV). Compared to clinically isolated strains, the vaccine strains contain several non-synonymous mutations in open reading frames (ORFs) 0, 6, 31, 39, 55, 62, and 64. In particular, ORF62, encoding an immediate-early (IE) 62 protein that acts as a transactivator for viral gene expression, contains six non-synonymous mutations, but whether these mutations affect transactivation activity of IE62 is not understood. Read More

View Article and Full-Text PDF
June 2018

Cellular factors and IE62 activation of VZV promoters.

J Med Virol 2003 ;70 Suppl 1:S90-4

Department of Microbiology, 138 Farber Hall, University at Buffalo, Buffalo, NY 14214, USA.

The varicella zoster virus IE62 protein transactivates promoters representing all three kinetic classes of viral genes. This is accomplished in conjunction with the general transcription machinery of the cell and specific cellular transcription factors that act at sites located within viral promoters. The incidence of a subset of specific recognition sites was examined for the ubiquitous cellular factors Sp1 and USF within the VZV genome. Read More

View Article and Full-Text PDF
June 2003