A targeted/non-targeted screening method for perfluoroalkyl carboxylic acids and sulfonates in whole fish using quadrupole time-of-flight mass spectrometry and MS(e).

Anal Bioanal Chem 2014 Feb 19;406(5):1471-80. Epub 2013 Dec 19.

Department of Civil and Environmental Engineering, Clarkson University, 8 Clarkson Ave, Potsdam, NY, 13699, USA,

A new method for measuring perfluoroalkyl contaminants (PFCs) in biological matrices has been developed. An ultra-high pressure liquid chromatograph equipped with a quadrupole time-of-flight mass spectrometer (UPLC-QToF) was optimized using a continuous precursor/product ion monitoring mode. Unlike traditional targeted studies that isolate precursor/product ion pairs, the current method alternates between two ionization energy channels to continuously capture standard electrospray ionization (low energy) and collision induced dissociation (high energy) spectra. The result is the indiscriminant acquisition of paired low and high energy spectra for all constituents eluting from the chromatographic system. This technique was evaluated for the routine analysis of perfluoroalkyl species. Using this technique, linear perfluoroalkyl carboxylic acids (C4 to C14) and perfluoroalkyl sulfonates (C4, C6, C8 and C10) exhibited a linear range spanning over three orders of magnitude and were detectable at levels less than 1 pg on column with a root mean squared signal to noise ratio of 5 to 20. Lake trout (Salvelinus namaycush) and National Institutes of Standards and Technology Standard Reference Material 1946 were used to evaluate matrix effects and the accuracy of this method when applied to a whole fish extract. The current method was also evaluated as a diagnostic tool to identify unknown PFCs using experimental fragmentation patterns, mass defect filtering and Kendrick plots.

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http://link.springer.com/10.1007/s00216-013-7519-4
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http://dx.doi.org/10.1007/s00216-013-7519-4DOI Listing
February 2014
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References

(Supplied by CrossRef)

NL Stock et al.
2010

JP Benskin et al.
2010

JW Martin et al.
Environ Sci Technol 2004

M Houde et al.
Environ Sci Technol 2006

JP Benskin et al.
Anal Chem 2007

SPJ Leeuwen Van et al.
Environ Sci Technol 2006

SPJ Leeuwen Van et al.
J Chromatogr A 2009

A Jahnke et al.
J Chromatogr A 2009

U Berger et al.
J Chromatogr A 2005

J Verreault et al.
Environ Sci Technol 2007

R Guo et al.
Talanta 2008

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