Cell Rep 2013 Nov 31;5(3):839-48. Epub 2013 Oct 31.
Garvan Institute of Medical Research, Sydney NSW 2010, Australia; Institute for Molecular Bioscience, The University of Queensland, Brisbane QLD 4072, Australia.
Human mitochondrial DNA is transcribed as long polycistronic transcripts that encompass each strand of the genome and are processed subsequently into mature mRNAs, tRNAs, and rRNAs, necessitating widespread posttranscriptional regulation. Here, we establish methods for massively parallel sequencing and analyses of RNase-accessible regions of human mitochondrial RNA and thereby identify specific regions within mitochondrial transcripts that are bound by proteins. This approach provides a range of insights into the contribution of RNA-binding proteins to the regulation of mitochondrial gene expression.