ACS Macro Lett 2013 Aug;2(9)
Departments of Chemistry and Chemical Engineering, Texas A&M University, College Station, Texas 77842, United States.
Nat Commun 2021 03 3;12(1):1411. Epub 2021 Mar 3.
Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing, China.
Genetically programmed circuits allowing bifunctional dynamic regulation of enzyme expression have far-reaching significances for various bio-manufactural purposes. However, building a bio-switch with a post log-phase response and reversibility during scale-up bioprocesses is still a challenge in metabolic engineering due to the lack of robustness. Here, we report a robust thermosensitive bio-switch that enables stringent bidirectional control of gene expression over time and levels in living cells. Read More
Macromol Rapid Commun 2021 Mar 1:e2000746. Epub 2021 Mar 1.
Institute of Physical Chemistry, Universität, Hamburg, Martin-Luther-King-Platz 6, Hamburg, 20146, Germany.
The recovery of lithium from global water resources continues to be challenging due to interfering metal ions with similar solution properties. Hence, a lithium-selective diblock copolymer system containing crown ethers (CEs) is developed. A polystyrene-block-poly(methacrylic acid) diblock copolymer is synthesized first via a one-pot solution-emulsion reversible addition-fragmentation chain transfer polymerization. Read More
Macromol Rapid Commun 2021 Mar 1:e2100027. Epub 2021 Mar 1.
Institute of Organic Chemistry, Karlsruhe Institute of Technology, Fritz-Haber-Weg 6, Karlsruhe, 76131, Germany.
Ring-opening metathesis polymerization is a robust method to synthesize a variety of polymers by using ring-strained molecules as monomers, e.g., norbornenes. Read More
Biophys J 2021 Feb 20. Epub 2021 Feb 20.
Department of Physics; Department of Bioengineering; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts. Electronic address:
The use of chaotropic reagents is common in biophysical characterization of biomolecules. When the study involves transmembrane protein channels, the stability of the protein channel and supporting bilayer membrane must be considered. In this letter, we show that planar bilayers composed of poly(1,2-butadiene)-b-poly(ethylene oxide) diblock copolymer are stable and leak-free at high guanidinium chloride concentrations, in contrast to diphytanoyl phosphatidylcholine bilayers, which exhibit deleterious leakage under similar conditions. Read More
Macromol Biosci 2021 Feb 22:e2000371. Epub 2021 Feb 22.
Laboratory for Chemical Design of Bionanomaterials, Faculty of Chemistry, M. V. Lomonosov Moscow State University, 1 Leninskie Gory, Moscow, 117234, Russia.
Macrophages are desirable targets for gene therapy of cancer and other diseases. Cationic diblock copolymers of polyethylene glycol (PEG) and poly-L-lysine (PLL) or poly{N-[N-(2-aminoethyl)-2-aminoethyl]aspartamide} (pAsp(DET)) are synthesized and used to form polyplexes with a plasmid DNA (pDNA) that are decorated with mannose moieties, serving as the targeting ligands for the C type lectin receptors displayed at the surface of macrophages. The PEG-b-PLL copolymers are known for its cytotoxicity, so PEG-b-PLL-based polyplexes are cross-linked using reducible reagent dithiobis(succinimidyl propionate) (DSP). Read More