Vibrio cholerae porin OmpU induces pro-inflammatory responses, but down-regulates LPS-mediated effects in RAW 264.7, THP-1 and human PBMCs.

Authors:
Sanica C Sakharwade
Sanica C Sakharwade
Manipal University
India
Praveen K Sharma
Praveen K Sharma
159 Goessmann Laboratory
United States
Arunika Mukhopadhaya
Arunika Mukhopadhaya
Albert Einstein College of Medicine
United States

PLoS One 2013 27;8(9):e76583. Epub 2013 Sep 27.

Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Mohali, Mohali, Punjab, India.

Vibrio cholerae porin OmpU plays a crucial role in the survival of the organism in the human gut. Various observations suggest critical involvement of OmpU in V. cholerae pathogenesis. However, OmpU is poorly characterized in terms of its ability to evoke cellular responses, particularly in the context of host immune system. Therefore, towards characterizing V. cholerae OmpU for its host immunomodulatory functions, we have studied the ability of OmpU to elicit pro-inflammatory responses in a range of immune cells which include, mouse RAW 264.7 macrophages, human THP-1 monocytes and human PBMCs. We have observed that purified OmpU induces pro-inflammatory responses in terms of production of NO, TNFα and IL-6. Interestingly, pre-treatment of the cells with OmpU suppresses the production of NO, TNFα, IL-6 as well as IL-12 upon subsequent activation with LPS. Our results therefore suggest that V. cholerae OmpU may have a differential regulatory role in terms of host immunomodulatory function: it can induce pro-inflammatory responses in target host immune cells, whereas it can also exert suppressive effect on LPS-induced pro-inflammatory responses. In addition, our study indicates that purified OmpU may have the ability to skew the Th1 response towards the Th2 response, presumably via suppression of IL-12 production.

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0076583PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3785423PMC
June 2014
23 Reads

Publication Analysis

Top Keywords

pro-inflammatory responses
20
ompu
10
immune cells
8
host immune
8
human pbmcs
8
purified ompu
8
production tnfα
8
host immunomodulatory
8
tnfα il-6
8
raw 2647
8
cholerae ompu
8
cholerae porin
8
porin ompu
8
vibrio cholerae
8
ompu induces
8
induces pro-inflammatory
8
responses
6
pro-inflammatory
5
responses terms
4
observed purified
4

Similar Publications