Fluidic automation of nitrate and nitrite bioassays in whole blood by dissolvable-film based centrifugo-pneumatic actuation.

Authors:
Charles E Nwankire
Charles E Nwankire
Dublin City University
Ireland
Di-Sien S Chan
Di-Sien S Chan
Dublin City University
Ireland
Robert Burger
Robert Burger
University of California
United States
Robert Gorkin
Robert Gorkin
Dublin City University
Ireland
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Fraunhofer Project Centre at Dublin City University
Professor (Full)
microfluidics, Lab-on-a-Chip, hydrodynanmics, business development, project management, organisational leadership
Glasnevin, Dublin 9 | Ireland

Sensors (Basel) 2013 Aug 26;13(9):11336-49. Epub 2013 Aug 26.

Biomedical Diagnostics Institute, National Centre for Sensor Research, School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.

This paper demonstrates the full centrifugal microfluidic integration and automation of all liquid handling steps of a 7-step fluorescence-linked immunosorbent assay (FLISA) for quantifying nitrate and nitrite levels in whole blood within about 15 min. The assay protocol encompasses the extraction of metered plasma, the controlled release of sample and reagents (enzymes, co-factors and fluorescent labels), and incubation and detection steps. Flow control is implemented by a rotationally actuated dissolvable film (DF) valving scheme. In the valves, the burst pressure is primarily determined by the radial position, geometry and volume of the valve chamber and its inlet channel and can thus be individually tuned over an extraordinarily wide range of equivalent spin rates between 1,000 RPM and 5,500 RPM. Furthermore, the vapour barrier properties of the DF valves are investigated in this paper in order to further show the potential for commercially relevant on-board storage of liquid reagents during shelf-life of bioanalytical, ready-to-use discs.

Download full-text PDF

Source
http://dx.doi.org/10.3390/s130911336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821360PMC

Still can't find the full text of the article?

Sign up to send a request to the authors directly.
August 2013
38 Reads
2.25 Impact Factor

Publication Analysis

Top Keywords

nitrate nitrite
8
fluorescent labels
4
rpm vapour
4
co-factors fluorescent
4
vapour barrier
4
enzymes co-factors
4
labels incubation
4
incubation detection
4
steps flow
4
flow control
4
rpm 5500
4
detection steps
4
reagents enzymes
4
5500 rpm
4
release sample
4
investigated paper
4
channel individually
4
encompasses extraction
4
protocol encompasses
4
paper order
4

Similar Publications

Inflammation and psychopathology in children following PICU admission: an exploratory study.

Evid Based Ment Health 2018 Nov 9;21(4):139-144. Epub 2018 Oct 9.

Department of Paediatric Intensive Care, St Mary's Hospital, Imperial College Healthcare NHS Trust, London, UK.

Background: Survivors of critical illness in childhood commonly display subsequent psychiatric symptoms including emotional and behavioural difficulties, and manifestations of post-traumatic stress disorder (PTSD). Anomalies in inflammatory profiles are an established finding in these childhood psychiatric conditions.

Objective: This exploratory study aimed to investigate whether abnormal peripheral blood inflammatory markers measured during paediatric intensive care unit (PICU) admission were associated with psychiatric symptoms after discharge. Read More

View Article
November 2018
3 Reads

Ferrocenyl-Coupled N-Heterocyclic Carbene Complexes of Gold(I): A Successful Approach to Multinuclear Anticancer Drugs.

Chemistry 2016 Dec 17;22(52):18953-18962. Epub 2016 Nov 17.

Organic Chemistry Laboratory, University Bayreuth, Universitätsstrasse 30, 95447, Bayreuth, Germany.

Four gold(I) carbene complexes featuring 4-ferrocenyl-substituted imidazol-2-ylidene ligands were investigated for antiproliferative and antivascular properties. They were active against a panel of seven cancer cell lines, including multidrug-resistant ones, with low micromolar or nanomolar IC (72 h) values, according to their lipophilicity and cellular uptake. The delocalized lipophilic cationic complexes 8 and 10 acted by increasing the reactive oxygen species in two ways: through a genuine ferrocene effect and by inhibiting the thioredoxin reductase. Read More

View Article
December 2016
14 Reads
2 PubMed Central Citations(source)
5.73 Impact Factor

Ablation of CD11c(hi) dendritic cells exacerbates Japanese encephalitis by regulating blood-brain barrier permeability and altering tight junction/adhesion molecules.

Comp Immunol Microbiol Infect Dis 2016 Oct 26;48:22-32. Epub 2016 Jul 26.

College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Iksan 54596, Republic of Korea; Department of Bioactive Material Sciences, Graduate School, Chonbuk National University, Jeonju 54896, Republic of Korea. Electronic address:

Japanese encephalitis (JE), characterized by extensive neuroinflammation following infection with neurotropic JE virus (JEV), is becoming a leading cause of viral encephalitis due to rapid changes in climate and demography. The blood-brain barrier (BBB) plays an important role in restricting neuroinvasion of peripheral leukocytes and virus, thereby regulating the progression of viral encephalitis. In this study, we explored the role of CD11c(hi) dendritic cells (DCs) in regulating BBB integrity and JE progression using a conditional depletion model of CD11c(hi) DCs. Read More

View Article
October 2016
18 Reads
4 PubMed Central Citations(source)
2.02 Impact Factor

A new scale for disaster nursing core competencies: Development and psychometric testing.

Australas Emerg Nurs J 2016 Feb 14;19(1):11-9. Epub 2016 Jan 14.

School of Nursing & Midwifery, Monash University, Australia; Nursing Research, Monash University and Peninsula Health, Australia. Electronic address:

Background: All nurses must have core competencies in preparing for, responding to and recovering from a disaster. In the Kingdom of Saudi Arabia (KSA), as in many other countries, disaster nursing core competencies are not fully understood and lack reliable, validated tools. Thus, it is imperative to develop a scale for exploring disaster nursing core competencies, roles and barriers in the KSA. Read More

View Article
February 2016
27 Reads

A phase I/II study of erlotinib in combination with the anti-insulin-like growth factor-1 receptor monoclonal antibody IMC-A12 (cixutumumab) in patients with advanced non-small cell lung cancer.

J Thorac Oncol 2012 Feb;7(2):419-26

University of Colorado Cancer Center, Aurora, Colorado 80045, USA.

Introduction: This phase I/II study evaluated the safety and antitumor effect of the combination of erlotinib with cixutumumab, a recombinant fully humanized anti-insulin-like growth factor-1 receptor IgG1 monoclonal antibody, in advanced non-small cell lung cancer (NSCLC).

Methods: Patients with advanced NSCLC were treated in an initial safety-lead and drop-down cohorts using erlotinib 150 mg/d with cixutumumab 6 or 5 mg/kg on days 1, 8, 15, and 22 in 28-day cycles (cohorts 1 and 2). Emerging pharmacokinetic data led to an additional cohort (3 + 3 design) with cixutumumab at 15 mg/kg on day 1 in 21-day cycles (cohort 3). Read More

View Article
February 2012
7 Reads
27 PubMed Central Citations(source)
5.28 Impact Factor