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Diversity of Δ12 fatty acid desaturases in santalaceae and their role in production of seed oil acetylenic fatty acids. | PubFacts

Diversity of Δ12 fatty acid desaturases in santalaceae and their role in production of seed oil acetylenic fatty acids.

Authors:
Shoko Okada
Shoko Okada
Tokyo Dental College
Japan
Xue-Rong Zhou
Xue-Rong Zhou
Australia ; Biotechnology Research Institute/The National Key Facility for Crop Gene Resources and Genetic Improvement
China
Nerida Gibb
Nerida Gibb
CSIRO Ecosystem Sciences
Craig Wood
Craig Wood
National Water Research Institute
Canada
Mats Hamberg
Mats Hamberg
Karolinska Institutet
Sweden
Victoria S Haritos
Victoria S Haritos
Monash University
Australia

J Biol Chem 2013 Nov 23;288(45):32405-13. Epub 2013 Sep 23.

From the Commonwealth Scientific and Industrial Research Organization (CSIRO) Ecosystem Sciences, GPO Box 1700, Canberra, Australian Capital Territory 2601, Australia.

Plants in the Santalaceae family, including the native cherry Exocarpos cupressiformis and sweet quandong Santalum acuminatum, accumulate ximenynic acid (trans-11-octadecen-9-ynoic acid) in their seed oil and conjugated polyacetylenic fatty acids in root tissue. Twelve full-length genes coding for microsomal Δ12 fatty acid desaturases (FADs) from the two Santalaceae species were identified by degenerate PCR. Phylogenetic analysis of the predicted amino acid sequences placed five Santalaceae FADs with Δ12 FADs, which include Arabidopsis thaliana FAD2. When expressed in yeast, the major activity of these genes was Δ12 desaturation of oleic acid, but unusual activities were also observed: i.e. Δ15 desaturation of linoleic acid as well as trans-Δ12 and trans-Δ11 desaturations of stearolic acid (9-octadecynoic acid). The trans-12-octadecen-9-ynoic acid product was also detected in quandong seed oil. The two other FAD groups (FADX and FADY) were present in both species; in a phylogenetic tree of microsomal FAD enzymes, FADX and FADY formed a unique clade, suggesting that are highly divergent. The FADX group enzymes had no detectable Δ12 FAD activity but instead catalyzed cis-Δ13 desaturation of stearolic acid when expressed in yeast. No products were detected for the FADY group when expressed recombinantly. Quantitative PCR analysis showed that the FADY genes were expressed in leaf rather than developing seed of the native cherry. FADs with promiscuous and unique activities have been identified in Santalaceae and explain the origin of some of the unusual lipids found in this plant family.

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Source
http://dx.doi.org/10.1074/jbc.M113.511931DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3820875PMC
November 2013
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