Toxoplasma transcription factor TgAP2XI-5 regulates the expression of genes involved in parasite virulence and host invasion.

J Biol Chem 2013 Oct 10;288(43):31127-38. Epub 2013 Sep 10.

From the Center for Infection and Immunity of Lille, CNRS UMR 8204, INSERM 1019, Université Lille Nord de France, Institut Pasteur de Lille, 59 000 Lille, France.

Gene regulation in apicomplexan parasites, a phylum containing important protozoan parasites such as Plasmodium and Toxoplasma, is poorly understood. The life cycle of Toxoplasma gondii is complex, with multiple proliferation and differentiation steps, of which tachyzoite proliferation is the most relevant to pathogenesis in humans and animals. Tachyzoites express invasion and virulence factors that are crucial for their survival and manipulation of host cell functions. The expression of those factors is tightly controlled during the tachyzoite cell cycle to permit their correct packaging in newly formed apical secretory organelles named micronemes and rhoptries in the daughter cells. However, little is known about the factors that control the expression of genes encoding the virulence factors present in these parasite-specific secretory organelles. We report that the plant-like nuclear factor TgAP2XI-5 targets more than 300 gene promoters and actively controls the transcription of these genes. Most of these target genes, including those that are essential for parasite virulence, showed a peak of expression in the S and M phases of the cell cycle. Furthermore, we identified the cis-regulatory element recognized by TgAP2XI-5 and demonstrated its ability to actively drive gene transcription. Our results demonstrated that TgAP2XI-5 is a novel DNA sequence-specific transcription factor associated with promoter activation. TgAP2XI-5 may regulate gene transcription of crucial virulence factors in T. gondii.

Download full-text PDF

Source
http://dx.doi.org/10.1074/jbc.M113.486589DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829425PMC
October 2013
10 Reads

Publication Analysis

Top Keywords

virulence factors
12
cell cycle
8
parasite virulence
8
secretory organelles
8
expression genes
8
gene transcription
8
transcription factor
8
factor tgap2xi-5
8
tgap2xi-5
5
factors
5
virulence
5
genes encoding
4
encoding virulence
4
factors parasite-specific
4
control expression
4
factors control
4
daughter cells
4
cells factors
4
parasite-specific secretory
4
organelles report
4

References

(Supplied by CrossRef)

Similar Publications