The effects of preoperative immunosuppressive therapy on ischemia and reperfusion (I/R) injury in healthy rats.

Int Urol Nephrol 2014 Feb 8;46(2):389-93. Epub 2013 Sep 8.

─░zmir Tepecik Training and Research Hospital Transplant Department, Izmir, Turkey.

Purpose: Warm-ischemia-induced injuries might be encountered during renal transplants from cadavers and healthy donors. Toll-like receptors (TLR) in ischemia-reperfusion (I/R) injury are one of the indicators of intracellular injury pathways. The intensity of ischemic injury is directly proportionate to high TLR levels. To minimize the I/R injury, we investigated TLR2 and TLR4 levels on rats, which were pretreated with tacrolimus (FK506) before I/R.

Methods: Eight Wistar albino rats in the study group were administered .01 mg/kg intramuscular tacrolimus. Administration to the study group was performed 24 and 1 h before warm ischemia. Eight rats in the control group were injected with 0.1 c.c. of distilled water. Blood samples were collected from the tail veins of all the rats on the first, second and third days. Expression levels of TLR2 and TLR4 genes were analyzed using the polymerase chain reaction method, to determine any significant difference between the control and study groups on the days when blood was taken.

Results: TLR2 (p = 0.045) and TLR4 (p = 0.022) levels in the study group were found to be statistically, and significantly, lower than those in the control group, on the second day following warm-ischemia- and reperfusion-induced injury.

Conclusions: Administration of immunosuppressive drugs to healthy donor rats led to a statistically significant reduction in the expression levels of TLR2 and TLR4 in the early period. In light of the data obtained by this study, we hypothesize that a preoperative therapy on donors might have a role in preventing I/R injury.

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11255-013-0548-2DOI Listing
February 2014
18 Reads

Publication Analysis

Top Keywords

i/r injury
16
study group
12
tlr2 tlr4
12
expression levels
8
control group
8
levels tlr2
8
rats
6
injury
6
study
5
levels
5
group
5
rats study
4
albino rats
4
0045 tlr4
4
tlr2 0045
4
intramuscular tacrolimus
4
blood takenresults
4
mg/kg intramuscular
4
takenresults tlr2
4
wistar albino
4

References

(Supplied by CrossRef)

DK Dhar et al.
Transplantation 1992

BS Kim et al.
Transplantation 2005

S Hayashi et al.
J Heart Lung Transpl 1995

LA Valdivia et al.
Transplantation 1987

LA Valdivia et al.
Transplantation 1990

Y Yamaguchi et al.
Transplantation 1990

S Akira et al.
Nat Immunol 2001

HD Brightbill et al.
Science 1999

MA Dobrovolskaia et al.
J Immunol 2003

T Horng et al.
Nat Immunol 2001

N Silverman et al.
Genes Dev 2001

Similar Publications