Leishmania braziliensis: cytotoxic, cytostatic and chemotactic effects of poly-lysine-methotrexate-conjugates.

Authors:
Emilia Diaz
Emilia Diaz
Kaitek Labs
Arturo Rios
Arturo Rios
Department of Innovation and Technological Development
Chicago | United States
Rita Szabo
Rita Szabo
National Center for Epidemiology
Ferenc Hudecz
Ferenc Hudecz
Eötvös Loránd University

Exp Parasitol 2013 Sep 28;135(1):134-41. Epub 2013 Jun 28.

Laboratorio de Fisiología Molecular, Instituto de Medicina Experimental, Escuela Luis Razetti, Facultad de Medicina, Universidad Central de Venezuela, Venezuela.

Chemotactic responses play a significant role during Leishmania differentiation, as well as in the course of parasite-host-cell interaction, a process that precedes a successful infection. The present study uses the modified "two-chamber capillary assay" to quantitatively evaluate the chemotactic properties and the toxic activities of methotrexate containing branched chain polymeric polypeptide conjugates in Leishmania. Our results demonstrate that this methodology quantitatively determines the taxis of Leishmania towards/against gradients of compounds. They also demonstrate that chemotaxis produced by the polypeptide-methotrexate conjugates depends on specific chemical characteristics. For example, the N-terminal amino acid (Ser or Glu) location at the branch significantly influences the elicited chemotaxis. Furthermore, the use of different attachment sites in the methotrexate conjugates (α- or γ-carboxylic groups) affect their chemotactic activity. Specific cytotoxic activities and cytostatic effects of the conjugates on parasites and on murine and human cells of the macrophage/monocyte system respectively, suggest that these ligands may be used as a group of anti-Leishmania substances acting selectively on Leishmania and different hosts.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.exppara.2013.06.007DOI Listing

Still can't find the full text of the article?

Sign up to send a request to the authors directly.
September 2013
20 Reads

Publication Analysis

Top Keywords

leishmania
5
chemical characteristics
4
characteristics example
4
specific chemical
4
depends specific
4
conjugates depends
4
example n-terminal
4
polypeptide-methotrexate conjugates
4
n-terminal amino
4
location branch
4
branch influences
4
glu location
4
ser glu
4
amino acid
4
acid ser
4
produced polypeptide-methotrexate
4
demonstrate chemotaxis
4
leishmania demonstrate
4
demonstrate methodology
4
conjugates leishmania
4

Similar Publications

Circular Keratotomy Combined With Wedge Resection in the Management of High Astigmatism After Penetrating Keratoplasty.

Eye Contact Lens 2018 Nov;44 Suppl 2:S392-S395

Department of Ophthalmology (H.Ç.), Türkiye Hospital, Eye Clinic, İstanbul, Turkey; Department of Ophthalmology (S.G.), Prof. Dr. N. Reşat Belger Beyoğlu Eye Training and Research Hospital, İstanbul, Turkey; and Department of Ophthalmology (E.G.), Medipol University Medical School, İstanbul, Turkey.

Objective: To evaluate the effectiveness of circular keratotomy combined with wedge resection for the management of high astigmatism after penetrating keratoplasty (PK).

Methods: The study included seven eyes of seven patients with previous PK who underwent circular keratotomy combined with wedge resection. The uncorrected visual acuity (UCVA) and best-corrected visual acuity (BCVA), spherical equivalent (SE) refraction, and keratometric powers obtained by corneal topography were evaluated. Read More

View Article
November 2018
29 Reads
1.68 Impact Factor

The role of inflammatory cytokines and tumor associated macrophages (TAMs) in microenvironment of pancreatic cancer.

Cytokine Growth Factor Rev 2018 Feb 12;39:46-61. Epub 2018 Jan 12.

Research Center for Immunodeficiencies, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Cancer Immunology Project (CIP), Universal Scientific Education and Research Network (USERN), Sheffield, UK. Electronic address:

Pancreatic cancer is considered as one of the most lethal types of cancer due to its poor prognosis and lack of effective therapeutic approaches. Although many studies have been done on pancreatic cancer, the current treatment methods did not exhibit successful results. Hence, novel strategies are needed for treatment of pancreatic cancer. Read More

View Article
February 2018
11 Reads
5.36 Impact Factor

Sex-Differences, Handedness, and Lateralization in the Iowa Gambling Task.

Authors:
Varsha Singh

Front Psychol 2016 31;7:708. Epub 2016 May 31.

Humanities and Social Science, Indian Institute of Technology Delhi New Delhi, India.

In a widely used decision-making task, the Iowa Gambling Task (IGT), male performance is observed to be superior to that of females, and is attributed to right lateralization (i.e., right hemispheric dominance). Read More

View Article
June 2016
31 Reads

Electromagnetic field therapy delays cellular senescence and death by enhancement of the heat shock response.

Exp Gerontol 2008 Apr 29;43(4):307-16. Epub 2008 Jan 29.

Department of Medicine and Section of Geriatric Medicine, University of Illinois Mail Code 717, College of Medicine, Chicago, IL 60612, USA.

Hormesis may result when mild repetitive stress increases cellular defense against diverse injuries. This process may also extend in vitro cellular proliferative life span as well as delay and reverse some of the age-dependent changes in both replicative and non-replicative cells. This study evaluated the potential hormetic effect of non-thermal repetitive electromagnetic field shock (REMFS) and its impact on cellular aging and mortality in primary human T lymphocytes and fibroblast cell lines. Read More

View Article
April 2008
12 Reads
8 PubMed Central Citations(source)
3.48 Impact Factor