Ethanolic extract of Alternanthera sessilis (AS-1) inhibits IgE-mediated allergic response in RBL-2H3 cells.

Authors:
Sheikh Rayees
Sheikh Rayees
Indian Institute of Integrative Medicine
India
Dr Ajay Kumar, PhD
Dr Ajay Kumar, PhD
ICGEB, New Delhi
Scientist
New Delhi, Delhi | India
Shafaq Rasool
Shafaq Rasool
LB Genetik University of Kaiserslautern
Germany
Peerzada Kaiser
Peerzada Kaiser
Indian Institute of Integrative Medicine
India
Payare Lal Sangwan
Payare Lal Sangwan
Indian Institute of Integrative Medicine
India
Surjeet Singh
Surjeet Singh
CSIR-Indian Institute of Integrative Medicine
New Delhi | India

Immunol Invest 2013 19;42(6):470-80. Epub 2013 Jun 19.

Pharmacology Division, Indian Institute of Integrative Medicine C.S.I.R., Jammu, India.

The present study was designed to investigate the anti-allergic effects of ethanolic extract of Alternanthera sessilis (AS-1) in rat basophilic leukemia (RBL-2H3) cells. It significantly reduced the β-hexosaminidase release from anti-DNP-IgE sensitized RBL-2H3 cells. AS-1also inhibited the IgE antibody-induced increase in Interleukin-6 (IL-6), TNF-α, IL-13 and IL-4 production in these cells. The inhibitory effect of AS-1 on these cytokine was found to be nuclear factor-KB (NF-kB) dependent, as it attenuated the degradation of IKBa and nuclear translocation of NFkB. In addition, AS-1 significantly attenuated the DNP HAS-induced intracellular Ca(2+) release from these cells, which makes us speculate strongly that the decreased intracellular Ca(2+) is involved in the inhibitory effect of AS-1 on β-hexoaminidase release. Taken together, anti-allergic effects of AS-1 suggest possible therapeutic application of this extract in allergic diseases.

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http://dx.doi.org/10.3109/08820139.2013.789909DOI Listing
February 2014
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