Effects of hyaluronic acid conjugation on anti-TNF-α inhibition of inflammation in burns.

J Biomed Mater Res A 2014 May 28;102(5):1527-36. Epub 2013 Jun 28.

Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania.

Biomaterials capable of neutralizing specific cytokines could form the basis for treating a broad range of conditions characterized by intense, local inflammation. Severe burns, spanning partial- to full-thickness of the dermis, can result in complications due to acute inflammation that contributes to burn progression, and early mediation may be a key factor in rescuing thermally injured tissue from secondary necrosis to improve healing outcomes. In this work, we examined the effects on burn progression and influence on the inflammatory microenvironment of topical application of anti-tumor necrosis factor-α (anti-TNF-α) alone, mixed with hyaluronic acid (HA) or conjugated to HA. We found that non-conjugated anti-TNF-α decreased macrophage infiltration to a greater extent than that conjugated to HA; however, there was little effect on the degree of progression or IL-1β levels. A simple transport model is proposed to analyze the results, which predicts qualitative and quantitative differences between untreated burn sites and those treated with the conjugates. Our results indicate that conjugation of anti-TNF-α to high molecular weight HA provides sustained, local modulation of the post-injury inflammatory responses compared to direct administration of non-conjugated antibodies.

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http://dx.doi.org/10.1002/jbm.a.34829DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4119967PMC
May 2014
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References

(Supplied by CrossRef)
The diagnosis of the depth of burning
Jackson et al.
Br J Surg 1953
Partial-thickness burns: Identification and management
Johnson et al.
Adv Skin Wound Care 2003
The pathogenesis of burn wound conversion
Singh et al.
Ann Plast Surg 2007
Pathophysiology of the burn wound and pharmacological treatment
Arturson et al.
The Rudi Hermans Lecture, 1995. Burns 1996
Toll-like receptor signalling
Akira et al.
Nat Rev Immunol 2004

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