BMC Biophys 2013 Jun 12;6. Epub 2013 Jun 12.
Functional Genomics Center Zurich, ETH Zurich/ University of Zurich, Winterthurerstrasse 190, Zurich, CH-8057, Switzerland.
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Biosens Bioelectron 2008 Jun 13;23(11):1694-700. Epub 2008 Feb 13.
Department of Food Engineering and Science, East China University of Science and Technology, Shanghai 200237, China.
A novel immobilization method based on oligonucleotide as linker has been developed for small molecule microarrays (SMMs) construction. The oligonucleotide tail was employed as a linker in solid-phase synthesis. Small molecules could be easily conjugated at the 5' end of the oligonucleotide, previously modified with a functional group. Read More
Adv Colloid Interface Sci 2004 Nov;111(1-2):3-27
Department of Chemical Engineering, Loughborough University, Loughborough LE11 3TU, UK.
In Section 1, spreading of small liquid drops over thin dry porous layers is investigated from both theoretical and experimental points of view [V.M. Starov, S. Read More
Methods Mol Biol 2016 ;1368:25-36
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznan, Poland.
The DNA microarray technique was supposed to help identifying and analyzing the expression level of tens of thousands of genes in the whole genome. But there is a serious problem concerning fabrication of the microarrays by chemical synthesis, such as specific and efficient linking of probes to a solid support. Therefore, we reckon that applying "click" chemistry to covalently anchor oligonucleotides on chemically modified supports may help construct microarrays in applications such as gene identification. Read More
Colloids Surf B Biointerfaces 2015 Apr 5;128:464-72. Epub 2015 Mar 5.
Immunoassay/Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, NCSR "Demokritos", GR-15310 Aghia Paraskevi, Greece. Electronic address:
A novel immobilization approach involving binding of preformed streptavidin/biotinylated oligonucleotide conjugates onto surfaces coated with biotinylated bovine serum albumin is presented. Microarrays prepared according to the proposed method were compared, in terms of detection sensitivity and specificity, with other immobilization schemes employing coupling of biotinylated oligonucleotides onto directly adsorbed surface streptavidin, or sequential coupling of streptavidin and biotinylated oligonucleotides onto a layer of adsorbed biotinylated bovine serum albumin. A comparison was performed employing biotinylated oligonucleotides corresponding to wild- and mutant-type sequences of seven single point mutations of the BRCA1 gene. Read More