The C-terminal flanking peptide of progastrin induces gastric cell apoptosis and stimulates colonic cell division in vivo.

Peptides 2013 Aug 3;46:83-93. Epub 2013 Jun 3.

Department of Surgery, University of Melbourne, Austin Health, Lance Townsend Building, Lvl 8, Studley Road, Heidelberg, Victoria, Australia 3084.

Progastrin (PG) is processed into a number of smaller peptides including amidated gastrin (Gamide), non-amidated glycine-extended gastrin (Ggly) and the C-terminal flanking peptide (CTFP). Several groups have reported that PG, Gamide and Ggly are biologically active in vitro and in vivo, and are involved in the development of gastrointestinal cancers. CTFP is bioactive in vitro but little is known of its effects in vivo. This study investigated the bioactivity of CTFP in vivo in normal tissues using gastrin deficient (GASKO) mice and in two mouse models of cancer (SCID mice bearing xenograft tumors expressing normal or knocked-down levels of gastrin and a mouse model of hepatic metastasis). As with Ggly, CTFP treatment stimulated colonic proliferation in GASKO mice compared to control. CTFP also significantly increased apoptosis in the gastric mucosa of male GASKO mice. CTFP did not appear to effect xenograft growth or the incidence of liver metastases. This is the first demonstration that CTFP has specific biological activity in vivo in the colon and stomach.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.peptides.2013.05.009DOI Listing
August 2013

Publication Analysis

Top Keywords

gasko mice
12
flanking peptide
8
c-terminal flanking
8
ctfp
7
vivo
5
mice bearing
4
scid mice
4
cancer scid
4
bearing xenograft
4
models cancer
4
tumors expressing
4
levels gastrin
4
gastrin mouse
4
mouse model
4
knocked-down levels
4
normal knocked-down
4
mouse models
4
expressing normal
4
xenograft tumors
4
mice
4

Similar Publications