Expression of Clostridium perfringens epsilon-beta fusion toxin gene in E. coli and its immunologic studies in mouse.

Reza Pilehchian Langroudi, Mehdi Shamsara, Khosrow Aghaiypour

Overview

Vaccination seems to be the most effective way to controlC. perfringens diseases. However, the industrial production ofClostridial toxins is laborious. Therefore, the production of tox-ins in heterologous expression systems is a viable alternative. Efficiency of vaccines based on Clostridium recombinant toxins has been reported . It has been shown that recombinant betaand epsilon toxins expressed in E. coli induce appropriate immuneresponses in mice and ruminants. In the present study, an epsilon-beta fusion toxin was expressed as asoluble protein in E. coli and the recombinant cell lysate was used for immunization studies in mouse.

Summary

Potency of the toxin (as an antigen) induced 6 and 10 IU/ml of epsilon and beta anti-toxin in rabbit,respectively. These titers were higher than the minimum level required by the European Pharmacopoeiafor epsilon and beta toxins. Experimental challenge with the recombinant fusion toxoid revealed that itcould protect mice against C. perfringens epsilon and beta toxins. Toxicity of the fusion toxin was studiedby histopathological findings, which were the same as the native toxins.

Author Comments

Dr. Reza Pilehchian Langroudi, PhD
Dr. Reza Pilehchian Langroudi, PhD
Razi vaccine and serum research institute
Molecular Genetics
Karaj, Alborz | Iran (Islamic Republic of)
This is the first report describing the expression of fusionepsilon-beta toxin in E. coli. Our findings suggest this fusion as acandidate for the development of vaccine against C. perfringens typeD and B.Dr. Reza Pilehchian Langroudi, PhD

Resources

http://archrazi.areeo.ac.ir/article_103859.html
http://archrazi.areeo.ac.ir/article_103859.html

Expression of Clostridium perfringens epsilon-beta fusion toxin gene in E. coli and its immunologic studies in mouse.

Authors:
Dr. Reza Pilehchian Langroudi, PhD
Dr. Reza Pilehchian Langroudi, PhD
Razi vaccine and serum research institute
Molecular Genetics
Karaj, Alborz | Iran (Islamic Republic of)

Vaccine 2013 Jul 23;31(32):3295-9. Epub 2013 May 23.

Department of Anaerobic Bacterial Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Karaj, Iran.

Clostridium perfringens is an anaerobic spore-forming, pathogenic bacterium that is responsible for severe diseases in humans and livestock. In the present study, an epsilon-beta fusion toxin was expressed as a soluble protein in E. coli and the recombinant cell lysate was used for immunization studies in mouse. Potency of the toxin (as an antigen) induced 6 and 10IU/ml of epsilon and beta anti-toxin in rabbit, respectively. These titers were higher than the minimum level required by the European Pharmacopoeia for epsilon and beta toxins. Experimental challenge with the recombinant fusion toxoid revealed that it could protect mice against C. perfringens epsilon and beta toxins. Toxicity of the fusion toxin was studied by histopathological findings, which were the same as the native toxins. In conclusion, E. coli is a suitable expression host for immunogenic epsilon-beta fusion toxin of C. perfringens.

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Source
http://dx.doi.org/10.1016/j.vaccine.2013.04.061DOI Listing
July 2013
80 Reads
3 Citations
3.624 Impact Factor

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