Anal Chim Acta 2013 Jun 18;781:54-62. Epub 2013 Apr 18.
Biomedical Diagnostics Institute, National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland.
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Rev Sci Instrum 2011 Jun;82(6):064303
Department of Mechanical and Aerospace Engineering, University of California, Irvine, 4200 Engineering Gateway, Irvine, California 92697-3975, USA.
A novel, centrifugal disk-based micro-total analysis system (μTAS) for low cost and high throughput semi-automated immunoassay processing was developed. A key innovation in the disposable immunoassay disk design is in a fluidic structure that enables very efficient micro-mixing based on a reciprocating mechanism in which centrifugal acceleration acting upon a liquid element first generates and stores pneumatic energy that is then released by a reduction of the centrifugal acceleration, resulting in a reversal of direction of flow of the liquid. Through an alternating sequence of high and low centrifugal acceleration, the system reciprocates the flow of liquid within the disk to maximize incubation/hybridization efficiency between antibodies and antigen macromolecules during the incubation/hybridization stage of the assay. Read More
Biosens Bioelectron 2015 Jun 30;68:382-389. Epub 2014 Dec 30.
Biomedical Diagnostics Institute, National Center for Sensor Research, Dublin City University, Ireland; School of Physical Sciences, Dublin City University, Ireland. Electronic address:
An electrochemical Lab-on-a-Disc (eLoaD) platform for the automated quantification of ovarian cancer cells (SKOV3) from whole blood is reported. This centrifugal microfluidic system combines complex sample handling, i.e. Read More
Lab Chip 2011 Sep 29;11(18):3106-12. Epub 2011 Jul 29.
Department of Chemistry, University of California, Berkeley, CA 94720, USA.
A microfluidic device for solid-phase immunoassays based on microparticle labeling is developed using microvalve-control structures for automated sample processing. Programmable microvalve control in a multilayer structure provides automated sample delivery, adjustable hydrodynamic washing and compatibility with a wide range of substrates. Capture antibodies are derivatized on glass surfaces within the processor using an APTES patterning method, and magnetic microspheres conjugated with a secondary detection antibody are used as labels in a capture-sandwich format. Read More
Electrophoresis 2009 Jul;30(14):2481-7
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.
In this study, we developed an integrated and automatic microfluidic immunoassay system, which comprised a multi-layer immunoassay microfluidic chip and a rotary confocal LIF scanner. The normally closed elastomeric microvalves were integrated on the multi-layer microfluidic chip to realize flexible reagents delivery for the rapid immunoassay. The various operations of immunoassay including coating, blocking and washing could be performed on a single chip with reduced reagents consumption and analysis time. Read More