Adipose tissue-derived stem cells attenuate acute lung injury through eNOS and eNOS-derived NO.

Authors:
Dr. Peng Gao
Dr. Peng Gao
EPFL
Research Associate
Chemistry
Sion | Switzerland
Xu Yang
Xu Yang
College of Life Sciences
China
Dr. Sylvanus Kampo, Ph.D., M.med., CRA., RN.
Dr. Sylvanus Kampo, Ph.D., M.med., CRA., RN.
First Affiliated Hospital of Dalian Medical University.
Ph.D. Student
Anesthesiology
Dalian , Liaoning | China
Qingping Wen
Qingping Wen
Liaoning University of Traditional Chinese Medicine
China

Int J Mol Med 2013 Jun 4;31(6):1313-8. Epub 2013 Apr 4.

Department of Anesthesiology, Dalian Medical University, Dalian, People's Republic of China.

Acute lung injury (ALI) is among the most common causes of mortality in intensive care units. Recent in vivo and in vitro studies have suggested that mesenchymal stem cells (MSCs) attenuate pulmonary edema and inflammatory factors, but the mechanisms of the effects of MSCs on pulmonary vascular function remain unknown. It is believed that nitric oxide (NO) and endothelial nitric oxide synthase (eNOS) play an essential role in the regulation of vascular function and homeostasis. In the present study, we investigated the effect of adipose tissue-derived stem cells (ADSCs) on pulmonary microvascular endothelial cells (PMVECs) and the lung in a lipopolysaccharide (LPS)-induced ALI model in vitro and in vivo. Our results showed that ADSCs were able to attenuate the severity of ALI and pulmonary edema. Increased expression of the eNOS protein was also observed in pulmonary PMVECs and in the lung following treatment with ADSCs. Furthermore, ADSCs increased the concentration of eNOS-derived NO to remodel ALI. The results suggest that ADSCs may be a promising candidate for ALI treatment through interaction with eNOS and eNOS-derived NO.

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http://dx.doi.org/10.3892/ijmm.2013.1328DOI Listing

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June 2013
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