[Effect of homoharringtonine on expression of NF-κB and BCL-2 proteins in K562 cells].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2013 Feb;21(1):78-81

Department of Hematology, Nanjing Medical University, Huaian, Jiangsu Province, China.

This study was aimed to investigate the effect of homoharringtonine (HHT) on K562 cell proliferation, apoptosis and expression of BCL-2 and NF-κB proteins. The cells proliferation was assayed with MTT method, the cell apoptosis, cell cycle and BCL-2 expression were analyzed with flow cytometry, NF-κB protein expression was detected with Western blot. The results showed that HHT concentration-dependently inhibited proliferation of K562 cells, the IC50 at 48 h was 43.89 ng/ml. Treated with HHT 10 ng/ml for 48 h, K562 cell apoptosis significantly increased, cell cycle was blocked at G0/G1, the expression level of BCL-2 and NF-κB proteins was lower than that in control group (P < 0.05). It is concluded that HHT may inhibit the proliferation of K562 cells, and down-regulating expression levels of BCL-2 and NF-κB may be one of its anti-CML mechanisms.

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http://dx.doi.org/10.7534/j.issn.1009-2137.2013.01.017DOI Listing
February 2013
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