Biodegradation of single-walled carbon nanotubes by eosinophil peroxidase.

Authors:
Alexandr A Kapralov
Alexandr A Kapralov
University of Pittsburgh
United States
Naveena Yanamala
Naveena Yanamala
University of Pittsburgh
Pittsburgh | United States
Weihong Feng
Weihong Feng
Institute of Chinese Materia Madica
Arjang Baygan
Arjang Baygan
Karolinska Institutet
Sweden
Benedict J Chambers
Benedict J Chambers
Center for Infectious Medicine
Kjell Hultenby
Kjell Hultenby
Karolinska Institutet
Sweden
Fei Ye
Fei Ye
Nanjing First Hospital
Nanjing Shi | China

Small 2013 Aug 27;9(16):2721-9, 2720. Epub 2013 Feb 27.

Division of Molecular Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Nobels Väg 13, Stockholm 17177, Sweden.

Eosinophil peroxidase (EPO) is one of the major oxidant-producing enzymes during inflammatory states in the human lung. The degradation of single-walled carbon nanotubes (SWCNTs) upon incubation with human EPO and H₂O₂ is reported. Biodegradation of SWCNTs is higher in the presence of NaBr, but neither EPO alone nor H₂O₂ alone caused the degradation of nanotubes. Molecular modeling reveals two binding sites for SWCNTs on EPO, one located at the proximal side (same side as the catalytic site) and the other on the distal side of EPO. The oxidized groups on SWCNTs in both cases are stabilized by electrostatic interactions with positively charged residues. Biodegradation of SWCNTs can also be executed in an ex vivo culture system using primary murine eosinophils stimulated to undergo degranulation. Biodegradation is proven by a range of methods including transmission electron microscopy, UV-visible-NIR spectroscopy, Raman spectroscopy, and confocal Raman imaging. Thus, human EPO (in vitro) and ex vivo activated eosinophils mediate biodegradation of SWCNTs: an observation that is relevant to pulmonary responses to these materials.

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.201202508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4039041PMC

Still can't find the full text of the article?

We can help you send a request to the authors directly.
August 2013
17 Reads
22 Citations
8.370 Impact Factor

Publication Analysis

Top Keywords

biodegradation swcnts
12
human epo
8
eosinophil peroxidase
8
carbon nanotubes
8
epo h₂o₂
8
single-walled carbon
8
swcnts
6
epo
6
biodegradation
5
spectroscopy confocal
4
side epo
4
distal side
4
groups swcnts
4
epo oxidized
4
oxidized groups
4
swcnts cases
4
site distal
4
raman spectroscopy
4
side catalytic
4
swcnts epo
4

Altmetric Statistics

References

(Supplied by CrossRef)
Article in Toxicol. Appl. Pharmacol.
Shvedova et al.
Toxicol. Appl. Pharmacol. 2012
Article in Environ. Sci. Technol.
Jia et al.
Environ. Sci. Technol. 2005
Article in J. Toxicol. Environ. Health Part A
Kisin et al.
J. Toxicol. Environ. Health Part A 2007
Article in Toxicol. Lett.
Kagan et al.
Toxicol. Lett. 2006
Article in Am. J. Physiol. Lung Cell Mol. Physiol.
Shvedova et al.
Am. J. Physiol. Lung Cell Mol. Physiol. 2005
Article in Am. J. Physiol. Lung Cell Mol. Physiol.
Shvedova et al.
Am. J. Physiol. Lung Cell Mol. Physiol. 2008
Article in Toxicol. Appl. Pharmacol.
Muller et al.
Toxicol. Appl. Pharmacol. 2005
Article in Toxicol. Sci.
Teeguarden et al.
Toxicol. Sci. 2011
Article in Toxicol. Appl. Pharmacol.
Inoue et al.
Toxicol. Appl. Pharmacol. 2009
Article in Toxicol. Sci.
Nygaard et al.
Toxicol. Sci. 2009
Article in Free Radic. Biol. Med.
Inoue et al.
Free Radic. Biol. Med. 2010

Similar Publications