Genotoxicity of dried Hoodia parviflora aerial parts.

Authors:
Barry Lynch
Barry Lynch
Australian National University
Australia
Annette Lau
Annette Lau
Queen's University
Canada
Nigel Baldwin
Nigel Baldwin
University of Toronto
Canada
Mark R Bauter
Mark R Bauter
Product Safety Laboratories
Palma Ann Marone
Palma Ann Marone
School of Pharmacy
United States

Food Chem Toxicol 2013 May 21;55:272-8. Epub 2013 Jan 21.

Intertek Cantox, Suite 308, 2233 Argentia Rd., Mississauga, Ontario, Canada L5N 2X7.

Hoodia parviflora is being developed commercially for use in weight loss food and dietary supplement products. Its effects are ascribed to a number of glycosides that have been shown to be present in plant extracts from several Hoodia species, the best known of which is H. gordonii. H. parviflora has been identified as an alternative to H. gordonii, and, as part of the process to develop H. parviflora, in vitro genotoxicity tests, as recommended by recent European Food Safety Authority guidance, were conducted on a dried powder preparation of H. parviflora aerial parts. The preparation was tested for reverse mutation at doses up to 5,000μg/plate in Salmonella typhimurium strains TA98, TA100, TA1535, and TA1537, and in Escherichia coli WP2 uvrA TA, both in the presence and in the absence of an exogenous source of metabolic activation (rat liver S9). In addition, the dried powder was evaluated in an in vitro cytotoxicity chromosome aberration assay using human lymphocytes. Test conditions included both a 4 (up to 2500μg/mg) and 44-h exposure period (up to 1000μg/mg) and the incorporation of an exogenous source of metabolic activation (4-h exposure only). H. parviflora dried powder was non-genotoxic in both in vitro assays.

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Source
http://dx.doi.org/10.1016/j.fct.2013.01.014DOI Listing
May 2013
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