Search Our Scientific Publications & Authors

Food Res Int 2021 Mar 18;141:110134. Epub 2021 Jan 18.

Institute of Agro-products Processing, Anhui Academy of Agricultural Science, Hefei 230031, China. Electronic address:

In this study, ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) combined with principal component analysis (PCA) were used to investigate the effects of process conditions on the profiles of carcinogenic and mutagenic heterocyclic aromatic amine (HAA) in the pork roasted at 175 °C, 200 °C, 225 °C and 250 °C for 10, 15, 20, 25, 30, 35 and 40 min. Twelve HAAs from four categories, including carboline (Norharman, Harman, and Phe-p-1), imidazopyridine (PhIP, 4'-OH-PhIP, DMIP, and 1,5,6-TMIP), imidazoquinoline (IQ, IQ [4,5-b], and MeIQ), and imidazoquinoxaline (MeIQx and 4,8-DiMeIQx), were detected, quantified and used to compose the HAA profiles in roasted pork. After being Analyzed by PCA, the distributions of HAA profiles from different temperature on the PCA score plot demonstrated that there are significant differences among the HAA profiles from different temperatures. Read More

Biochim Biophys Acta Proteins Proteom 2021 Feb 23:140634. Epub 2021 Feb 23.

Department of Biochemistry, School of Medicine, Tzu Chi University, Hualien 97004, Taiwan; Laboratory of Synthetic Biophysics and Chemical Biology, Biophysics Institute for Research and Development (BIRD), Chiang Mai 50230, Thailand; Bacterial Toxin Research Innovation Cluster (BRIC), Institute of Molecular Biosciences, Mahidol University, Salaya Campus, Nakornpathom 73170, Thailand. Electronic address:

One proposed toxic mechanism of Bacillus thuringiensis Cry δ-endotoxins involves pore formation in target membranes by the α4-α5 transmembrane hairpin constituting their pore-forming domain. Here, nine selected charged and uncharged polar residues in the pore-lining α4 of the Cry4Aa mosquito-active toxin were substituted with Ala. All mutant toxins, i. Read More

Am J Physiol Heart Circ Physiol 2021 Feb 26. Epub 2021 Feb 26.

Département de médecine, Centre de Recherche du CHUS, Institut de Pharmacologie de Sherbrooke, Faculté de Médecine et des Sciences de la Santé, Université de Sherbrooke, Sherbrooke, Québec, Canada.

Apelin-receptor (APJ) activation by apelin-13 (APLN-13) engages both Gαi proteins and β-arrestins, stimulating distinct intracellular pathways and triggering physiological responses like enhanced cardiac contractility. Substituting the C-terminal phenylalanine of APLN-13 with α-methyl-L-phenylalanine ((L-α-Me)Phe) or Benzoyl-L-phenylalanine (Bpa) generates biased analogues inducing APJ functional selectivity toward Gαi proteins. Using these original analogues, we proposed to investigate how the canonical Gαi signaling of APJ regulates the cardiac function, and to assess their therapeutic impact in a rat model of isoproterenol-induced myocardial dysfunction. Read More

ACS Chem Biol 2021 Feb 24. Epub 2021 Feb 24.

Department of Chemistry, Tokyo Institute of Technology, 2-12-1 Meguro-ku, O-okayama, Tokyo 152-8551, Japan.

Hitachimycin is a macrolactam antibiotic with an ()-β-phenylalanine (β-Phe) at the starter position of its polyketide skeleton. ()-β-Phe is formed from l-α-phenylalanine by the phenylananine-2,3-aminomutase HitA in the hitachimycin biosynthetic pathway. In this study, we produced new hitachimycin analogs via mutasynthesis by feeding various ()-β-Phe analogs to a Δ strain. Read More

Environ Mol Mutagen 2021 Feb 23. Epub 2021 Feb 23.

Department of Analytical, Environmental and Forensic Sciences, MRC-PHE Centre for Environment and Health, King's College London, London, UK.

TP53 harbours somatic mutations in more than half of human tumours with some showing characteristic mutation spectra that have been linked to environmental exposures. In bladder cancer, a unique distribution of mutations amongst several codons of TP53 has been hypothesised to be caused by environmental carcinogens including 4-aminobiphenyl (4-ABP). 4-ABP undergoes metabolic activation to N-hydroxy-4-aminobiphenyl (N-OH-4-ABP) and forms pre-mutagenic adducts in DNA, of which N-(deoxyguanosin-8-yl)-4-ABP (dG-C8-4-ABP) is the major one. Read More