Genetic diversity study of some medicinal plant accessions belong to Apiaceae family based on seed storage proteins patterns.

Authors:
Sayed Mohammad Masoumi
Sayed Mohammad Masoumi
Razi University
Danial Kahrizi
Danial Kahrizi
Razi University
Iran
Dr Hossein Rostami-Ahmadvandi, PhD
Dr Hossein Rostami-Ahmadvandi, PhD
Razi University
molecular genetic
molecular genetic- biotrch
Kermanshah, Kermanshah | Iran
Jahad Soorni
Jahad Soorni
Razi University
Iran
Sara Kiani
Sara Kiani
Yale College
New Haven | United States
Ali Mostafaie
Ali Mostafaie
Kermanshah University of Medical Sciences
Iran
Kheirollah Yari
Kheirollah Yari
Medical Biology Research Center
Beijing | China

Mol Biol Rep 2012 Dec 20;39(12):10361-5. Epub 2012 Oct 20.

Department of Natural Resources, Razi University, Kermanshah, Iran.

Cumin (Cuminum cyminum L.), Fennel (Foeniculum vulgare L.) and Longleaf (Falcaria vulgaris Bernh) that all belong to Apiaceae family as medicinal plants are very important in many countries. Study of genetic diversity for medicinal plant is important for researches in future. One of the methods to evaluate plant genetic diversity and classification of them is the electrophoresis of seed storage proteins. This research was conducted in order to evaluate seed protein variability in different Iranian Cumin, Fennel and Longleaf accessions and grouping them based on these proteins as a biochemical marker. For this purpose, the samples were first powdered in liquid nitrogen and seed protein was extracted with extraction buffer. Then total soluble proteins were resolved on 12.5 % sodium dodecyl sulphate polyacrylamide gel electrophoresis gels. The electrophoretic protein pattern showed 38 bands that were low polymorphism among the accessions. The result of cluster analysis showed that the accessions were classified in three groups (all 29 Cumin accessions in the first group, three Fennel ecotypes in second group and three Longleaf accessions in the last one).

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December 2012
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References

(Supplied by CrossRef)

VH Heywood et al.
1971

R Omidbaigi et al.
2007

M Kafi et al.
2003
Article in Prog Essent Oil Res
A Akgul et al.
Prog Essent Oil Res 1986
Article in Iran J Pharm Ther
M Khazaei et al.
Iran J Pharm Ther 2006
Article in Biochem Genet
M Rahimmalek et al.
Biochem Genet 2009
Article in Int Res J Biotechnol
M Kakaei et al.
Int Res J Biotechnol 2011
Article in Euphytica
M Ravi et al.
Euphytica 2003
Article in Asian J Agric Sci
U Inam et al.
Asian J Agric Sci 2010
Article in J Agric Sci Technol A
H Rostami-Ahmadvandi et al.
J Agric Sci Technol A 2011
Article in Afr J Biotechnol
M Shuaib et al.
Afr J Biotechnol 2007

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