Electrophoresis 2012 Nov 12;33(21):3187-94. Epub 2012 Oct 12.
Instituto de Microelectrónica de Barcelona, IMB-CNM-CSIC, Universitat Autónoma de Barcelona, Barcelona, Spain.
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Electrophoresis 2015 Aug 20;36(16):1894-904. Epub 2015 Jul 20.
Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
A novel microfluidic label-free bead-based metallothionein immunosensors was designed. To the surface of superparamagnetic agarose beads coated with protein A, polyclonal chicken IgY specifically recognizing metallothionein (MT) were immobilized via rabbit IgG. The Brdicka reaction was used for metallothionein detection in a microfluidic printed 3D chip. Read More
Bioanalysis 2009 Apr;1(1):31-6
Department of Chemistry, Creighton University, Omaha, NE 68178, USA.
Microfluidic devices allow for fast detection with little reagent consumption. They also offer portability and the ability to mimic biological systems. Currently, many microfluidic devices are a hybrid of the microchip components, such as flow channels and wells, and larger instrumentation. Read More
Sensors (Basel) 2012 6;12(8):10810-9. Epub 2012 Aug 6.
Center for Nanobio Integration & Convergence Engineering (NICE), National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-806, Korea.
Lab-on-a-chip can provide convenient and accurate diagnosis tools. In this paper, a plastic-based microfluidic immunosensor chip for the diagnosis of swine flu (H1N1) was developed by immobilizing hemagglutinin antigen on a gold surface using a genetically engineered polypeptide. A fluorescent dye-labeled antibody (Ab) was used for quantifying the concentration of Ab in the immunosensor chip using a fluorescent technique. Read More
J Nanosci Nanotechnol 2014 Sep;14(9):6528-38
This paper describes the assembly of a bioelectrode based on poly(3-aminophenol) and anti-troponin T antibody for recognition of troponin T, which is a specific biomarker for diagnosis of acute myocardial infarction. This disease causes loss of cellular components, allowing the output of molecules such as troponin T. This proteic component acts as biomarker for diagnosis of acute myocardial infarction due to their high sensitivity and specificity. Read More