Design and fabrication of a COP-based microfluidic chip: chronoamperometric detection of Troponin T.

Electrophoresis 2012 Nov 12;33(21):3187-94. Epub 2012 Oct 12.

Instituto de Microelectrónica de Barcelona, IMB-CNM-CSIC, Universitat Autónoma de Barcelona, Barcelona, Spain.

This work demonstrates the design and fabrication of an all cyclo-olefin polymer based microfluidic device capable of capturing magnetic beads and performing electrochemical detection in a series of gold electrodes. The size of chip is of a microscope slide and features six independent measuring cells for multianalyte detection purposes. The aim of this work is to show that rapid prototyping techniques can be instrumental in the development of novel bioassays, particularly in clinical diagnosis applications. We show the successful determination of troponin-T, a cardiac disease marker, in the clinically relevant range of 0.05-1.0 ng/mL. This methodology achieves a detection limit of 0.017 ng/mL in PBS solutions, and is capable of detecting less than 1 ng/mL in a 1:50 human serum dilution.

Download full-text PDF

Source
http://dx.doi.org/10.1002/elps.201200225DOI Listing
November 2012
40 Reads
3.030 Impact Factor

Publication Analysis

Top Keywords

design fabrication
8
instrumental development
4
development novel
4
techniques instrumental
4
prototyping techniques
4
rapid prototyping
4
novel bioassays
4
bioassays clinical
4
successful determination
4
applications successful
4
diagnosis applications
4
clinical diagnosis
4
work rapid
4
aim work
4
slide features
4
microscope slide
4
chip microscope
4
size chip
4
features independent
4
independent measuring
4

References

(Supplied by CrossRef)
Article in Eur. Heart J.
Dickstein et al.
Eur. Heart J. 2008
Article in Clin. Chem.
Morrow et al.
Clin. Chem. 2007
Article in Clin. Chim. Acta
Cameron et al.
Clin. Chim. Acta 2007
Article in Circulation
James et al.
Circulation 2003
Article in J. Int. Med.
Tello-Montoliu et al.
J. Int. Med. 2007
Article in Clin. Biochem.
McDonnell et al.
Clin. Biochem. 2009
Article in Clin, Biochem.
Christenson et al.
Clin, Biochem. 2009
Article in Lab Chip
Park et al.
Lab Chip 2007
Article in Clin. Chem.
Wilding et al.
Clin. Chem. 2010
Article in Anal. Chem.
Arora et al.
Anal. Chem. 2010
Article in Microfluid. Nanofluidics
Nunes et al.
Microfluid. Nanofluidics 2010

Similar Publications