Intra-individual variability of serum hepcidin-25 in haemodialysis patients using mass spectrometry and ELISA.

Nephrol Dial Transplant 2012 Oct 19;27(10):3923-9. Epub 2012 Jul 19.

Department of Nephrology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands.

Background: Measurement of serum hepcidin levels may provide a useful alternative to the current methods of determining iron status in chronic haemodialysis (HD) patients. However, the biological variability of this pivotal regulator of iron homeostasis is unclear, and the impact of inflammation, dialysis clearance and iron therapy on hepcidin variability has not been established.

Methods: Two independent studies in chronic HD patients were conducted; serum hepcidin levels were measured at the start of dialysis sessions in 20 UK patients and in 43 Dutch patients by mass spectrometry (MS). Samples from UK patients were also analysed by a competitive enzyme-linked immunosorbent assay (cELISA). Coefficient of variance (CV(1)) was calculated and potential factors affecting CV(1) were also examined.

Results: The median CV(1) (inter-quartile range) was 23% (17-28) for the UK MS, 26% (17-48) for the Dutch MS and 23% (17-39) for the UK cELISA. The CV(1) was similar in those patients receiving and those not receiving regular intravenous iron. The CV(1) was not associated with the degree of inflammation. Hepcidin levels were higher following an inter-dialytic period of 3 versus 2 days (P = 0.02).

Conclusions: These findings suggest considerable variability of serum hepcidin levels in HD patients. Inflammation and the use of iron did not impact on the degree of variability, and hepcidin levels were higher after an inter-dialytic period of 3 versus 2 days. These findings need to be taken into account in future studies assessing the utility of serum hepcidin as a guide to the use of iron or erythropoiesis-stimulating agents therapy.

Download full-text PDF

Source
http://dx.doi.org/10.1093/ndt/gfs164DOI Listing
October 2012
11 Reads

Publication Analysis

Top Keywords

hepcidin levels
20
serum hepcidin
16
patients mass
8
inter-dialytic period
8
higher inter-dialytic
8
mass spectrometry
8
levels higher
8
haemodialysis patients
8
patients
8
variability serum
8
period versus
8
versus days
8
hepcidin
7
iron
6
serum
5
cv1
5
levels
5
enzyme-linked immunosorbent
4
findings considerable
4
degree variability
4

Similar Publications