Partially hydrolyzed guar gum affects the expression of genes involved in host defense functions and cholesterol absorption in colonic mucosa of db/db male mice.

Authors:
Zenta Yasukawa
Zenta Yasukawa
Laboratory of Animal Cell Function
Yuji Naito
Yuji Naito
Kyoto Prefectural University of Medicine
Japan
Tomohisa Takagi
Tomohisa Takagi
Kyoto Prefectural University of Medicine
Japan
Katsura Mizushima
Katsura Mizushima
Graduate School of Medical Science
El Paso | United States
Makoto Tokunaga
Makoto Tokunaga
Graduate School of Science
Japan
Noriyuki Ishihara
Noriyuki Ishihara
Hiroshima Prefectural University
Japan
Lekh R Juneja
Lekh R Juneja
Institute of Food Science and Nutrition
Switzerland
Toshikazu Yoshikawa
Toshikazu Yoshikawa
Kyoto Prefectural University of Medicine
Japan

J Clin Biochem Nutr 2012 Jul 22;51(1):33-8. Epub 2011 Dec 22.

Nutrition Division, Taiyo Kagaku Co. Ltd., 1-3 Takaramachi, Yokkaichi 510-0844, Japan.

Biomedical evidence in the last 20 years has shown that the consumption of partially hydrolyzed guar gum may influence lipid and/or carbohydrate metabolism at many levels. Since intestine represents the first interface to interact with dietary partially hydrolyzed guar gum in vivo, we evaluated gene expression profiles in small intestinal mucosa of db/db mice fed with partially hydrolyzed guar gum in an effort to delineate its effect on the small intestine. DNA microarray and real-time PCR analyses were performed to evaluate the gene expression profiles in mice small intestinal mucosa. Among the 28,853 transcripts represented on the GeneChip® microarray, no more than 20 genes exhibited up- or down-regulation by 1.5-fold or more after four weeks following partially hydrolyzed guar gum consumption. No adverse effects were apparent. We detected up- or down-regulation of some genes known to be involved in host defense functions and cholesterol absorption.

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Source
http://dx.doi.org/10.3164/jcbn.11-104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3389045PMC

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July 2012
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