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J Dairy Sci 2021 Apr 7. Epub 2021 Apr 7.

Department of Morphology, Faculty of Veterinary, Universidad de Las Palmas de Gran Canaria, Arucas, 35413 Las Palmas, Spain.

New cases of blue cheese discoloration has led to recent research to identify the causal agent and factors that favor blue pigment appearing. Nonetheless, very few reports have described the source of contamination and the measurements to eradicate the microbiological source on cheese farms by determining the relation between blue discoloration on fresh cheese and the Pseudomonas fluorescens group. Thus, 60 samples from a cheese farm (cheese, equipment surfaces, tap water, and raw and pasteurized milk) were analyzed by phenotypical, MALDI-TOF, 16S rRNA sequencing and pulsed-field gel electrophoresis tests to determine the causal agent. Read More

Sci Rep 2021 Apr 7;11(1):7678. Epub 2021 Apr 7.

Department of Chemical Engineering, National Institute of Technology Rourkela, Rourkela, Odisha, 769008, India.

The current study investigates the decolorization of Indanthrene Blue RS dye and the optimization of process parameters needed for effective decolorization by the bacterial consortium. The pure culture of strain TS8, PMS, and NCH has been isolated from the textile wastewater sample collected from local textile processing units outlet and dye contaminated soil from Odisha, India. A bacterial consortium-BP of Bacillus flexus TS8 (BF), Proteus mirabilis PMS (PM), and Pseudomonas aeruginosa NCH (PA) were developed. Read More

Microb Cell Fact 2021 Apr 7;20(1):80. Epub 2021 Apr 7.

CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, No. 9 Section 4, Renmin Nan Road, Chengdu, 610041, Sichuan, People's Republic of China.

Background: Daqu is the most important fermentation starter for Chinese liquor, with large number of microbes and enzymes being openly enriched in the Daqu system over thousands of years. However, only a few enzymes have been analyzed with crude protein for total liquefying power and saccharifying power of Daqu. Therefore, the complex enzymatic system present in Daqu has not been completely characterized. Read More

Bioorg Chem 2021 Mar 26;112:104863. Epub 2021 Mar 26.

CHO Pharma Inc., 7F, Building C, No. 99, Ln. 130, Sec. 1, Academia Rd., Nangang Dist., Taipei City 115, Taiwan; Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan. Electronic address:

The chemoenzymatic remodeled monoclonal antidodies with well-defined glycan structure at the Fc domain display improved biological activities, such as ADCC and ADCP, and are more likely to yield a better safety profile by eliminating the non-human glycans derived from CHO cell culture. We covalently immobilize wild type endoglycosidase S (EndoS), fucosidase, and EndoS2 mutant on magnetic beads through a linker to efficiently generate homogeneous antibody glycoforms without additional purification step to remove endoglycosidase and fucosidase. We also used the biotinylated wild type EndoS2 and EndoS2 mutant in combination with covalently immobilized fucosidase on magnetic beads to allow the sequential removal of endoglycosidases and fucosidase for efficient glyco-engineering and isolation of antibodies without purifying deglycosylated antibody intermediate. Read More

J Health Pollut 2021 Mar 25;11(29):210302. Epub 2021 Feb 25.

Department of Cell Biology and Genetics, Environmental Biology Unit, Faculty of Science, University of Lagos, Akoka, Yaba, Lagos, Nigeria.

Background: In addition to improving soil fertility and crop production, earthworms have been found to be useful in the removal of contaminants from soil, known as vermiremediation. Previous studies on vermiremediation have focused primarily on organic wastes, with relatively less attention paid to inorganic contaminants. In addition, some basic terms used in environmental health studies have often not been properly clarified. Read More