Effect of triptolide on T-cell receptor beta variable gene mRNA expression in rats with collagen-induced arthritis.

Anat Rec (Hoboken) 2012 Jun 27;295(6):922-7. Epub 2012 Apr 27.

Department of Human Anatomy, Basic Medicine College, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.

Triptolide (TP) has been used in the treatment of rheumatoid arthritis (RA), but its mechanism of action is not understood. T-cell activation and associated release of cytokines appear to be major factors in the pathogenesis of RA. The overexpression of T-cell receptor (TCR) variable gene (V gene) fragments can cause the activation and infiltration of autoreactive T cells. This study examines the effects of TP on rats with collagen-induced arthritis (CIA). The levels of interleukin-10 (IL-10) in the serum were examined with ELISA. Compared to the CIA group, the levels of IL-10 were greater in the TP treatment group. Real-time quantitative polymerase chain reaction confirmed that the expression of TCR V beta (BV) 15 and TCR BV19 was increased in the CIA group, whereas in the TP treatment group, the expression was decreased. In this study, TP was found to enhance IL-10 levels and decrease the expression levels of TCR BV15 and TCR BV19. These changes might help explain the effectiveness of TP in the treatment of RA.

Download full-text PDF

Source
http://dx.doi.org/10.1002/ar.22479DOI Listing
June 2012
16 Reads

Publication Analysis

Top Keywords

variable gene
8
tcr bv19
8
cia group
8
treatment group
8
collagen-induced arthritis
8
rats collagen-induced
8
t-cell receptor
8
tcr
5
group levels
4
group
4
compared cia
4
il-10 greater
4
real-time quantitative
4
quantitative polymerase
4
group real-time
4
greater treatment
4
elisa compared
4
levels il-10
4
serum examined
4
study examines
4

Similar Publications