Will a peripheral blood (PB) sample yield the same diagnostic and prognostic cytogenetic data as the concomitant bone marrow (BM) in myelodysplasia?

Leuk Res 2012 Jul 25;36(7):832-40. Epub 2012 Apr 25.

Stanford Hospitals and Clinics, Palo Alto, CA 94304, USA.

In patients with myelodysplastic syndromes (MDS), chromosome anomalies are detected by conventional cytogenetic studies (CCS) and/or interphase fluorescence in situ hybridization (FISH) of bone marrow (BM) samples and provide prognostic and diagnostic information, which can direct therapy. Whether peripheral blood (PB) can be substituted for bone marrow in these cases and can provide the same information remains unknown. Concurrent BM and PB specimens collected from 100 patients with recently diagnosed MDS were studied using both CCS and FISH. While 68% of BM samples showed an abnormal karyotype by CCS, only 31% of PB samples were abnormal by CCS. In 12% of patients, FISH and CCS were discordant due to the inability of the FISH panel to detect all possible abnormalities. However, only one case (1%) had a cryptic abnormality detected by FISH. BM and PB FISH were discordant in 3% of cases, most likely due to the smaller clone size in PB vs. BM. While PB should not be substituted for BM at diagnosis, it is a viable alternative for monitoring patients using the appropriate FISH probe(s).

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.leukres.2012.03.013DOI Listing
July 2012
21 Reads

Publication Analysis

Top Keywords

bone marrow
12
samples abnormal
8
peripheral blood
8
fish
7
ccs
5
abnormal ccs
4
studied ccs
4
mds studied
4
diagnosed mds
4
ccs fish
4
fish 68%
4
abnormal karyotype
4
karyotype ccs
4
31% samples
4
patients diagnosed
4
68% samples
4
ccs 31%
4
collected 100
4
blood substituted
4
substituted bone
4

Similar Publications