BDMC33, A curcumin derivative suppresses inflammatory responses in macrophage-like cellular system: role of inhibition in NF-κB and MAPK signaling pathways.

Int J Mol Sci 2012 6;13(3):2985-3008. Epub 2012 Mar 6.

Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; E-Mail:

Our preliminary screening has shown that curcumin derivative BDMC33 [2,6-bis(2,5-dimethoxybenzylidene)cyclohexanone] exerted promising nitric oxide inhibitory activity in activated macrophages. However, the molecular basis and mechanism for its pharmacological action is yet to be elucidated. The aim of this study was to investigate the anti-inflammatory properties of BDMC33 and elucidate its underlying mechanism action in macrophage cells. Our current study demonstrated that BDMC33 inhibits the secretion of major pro-inflammatory mediators in stimulated macrophages, and includes NO, TNF-α and IL-1β through interference in both nuclear factor kappaB (NF-κB) and mitogen activator protein kinase (MAPK) signaling cascade in IFN-γ/LPS-stimulated macrophages. Moreover, BDMC33 also interrupted LPS signaling through inhibiting the surface expression of CD-14 accessory molecules. In addition, the inhibitory action of BDMC33 not only restricted the macrophages cell (RAW264.7), but also inhibited the secretion of NO and TNF-α in IFN-γ/LPS-challenged microglial cells (BV-2). The experimental data suggests the inflammatory action of BDMC33 on activated macrophage-like cellular systems, which could be used as a future therapeutic agent in the management of chronic inflammatory diseases.

Download full-text PDF

Source
http://www.mdpi.com/1422-0067/13/3/2985
Publisher Site
http://dx.doi.org/10.3390/ijms13032985DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317699PMC
September 2015

Publication Analysis

Top Keywords

action bdmc33
8
mapk signaling
8
curcumin derivative
8
macrophage-like cellular
8
bdmc33
7
nf-κb mitogen
4
mitogen activator
4
factor kappab
4
interference nuclear
4
nuclear factor
4
kappab nf-κb
4
activator protein
4
macrophages bdmc33
4
bdmc33 interrupted
4
interrupted lps
4
ifn-γ/lps-stimulated macrophages
4
cascade ifn-γ/lps-stimulated
4
protein kinase
4
kinase mapk
4
signaling cascade
4

Similar Publications