Search our Database of Scientific Publications and Authors

I’m looking for a

    Details and Download Full Text PDF:
    Array-based capture, distribution, counting and multiplexed assaying of beads on a centrifugal microfluidic platform.

    Lab Chip 2012 Apr 15;12(7):1289-95. Epub 2012 Feb 15.
    Biomedical Diagnostics Institute, National Centre for Sensor Research, School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.
    We present a novel centrifugal microfluidic platform for the highly efficient manipulation and analysis of particles for applications in bead-based assays. The platform uses an array of geometrical V-cup barriers to trap particles using stopped-flow sedimentation under highly reproducible hydrodynamic conditions. The impact parameters governing the occupancy distribution and capture efficiency of the arrayed traps are investigated. The unique, nearly 100% capture efficiency paired with the capability to establish sharply peaked, single occupancy distributions enables a novel, digital readout mode for color-multiplexed, particle-based assays with low-complexity instrumentation. The presented technology marks an essential step towards a versatile platform for the integration of bead- and cell-based biological assays.
    PDF Download - Full Text Link
    ( Please be advised that this article is hosted on an external website not affiliated with
    Source Status ListingPossible

    Similar Publications

    Centrifugal microfluidics for biomedical applications.
    Lab Chip 2010 Jul 28;10(14):1758-73. Epub 2010 May 28.
    University of California, Irvine, Irvine, CA 92697, USA.
    The centrifugal microfluidic platform has been a focus of academic and industrial research efforts for almost 40 years. Primarily targeting biomedical applications, a range of assays have been adapted on the system; however, the platform has found limited commercial success as a research or clinical tool. Nonetheless, new developments in centrifugal microfluidic technologies have the potential to establish wide-spread utilization of the platform. Read More
    A lipobead microarray assembled by particle entrapment in a microfluidic obstacle course and used for the display of cell membrane receptors.
    Lab Chip 2013 Aug;13(15):3041-60
    Levich Institute and Department of Chemical Engineering, The City College of the City University of New York, New York, New York 10031, USA.
    Platforms which can display cell membrane ligands and receptors as a microarray library of probes for screening against a target are essential tools in drug discovery, biomarker identification, and pathogen detection. Membrane receptors and ligands require their native bilayer environment to retain their selectivity and binding affinity, and this complicates displaying them in a microarray platform. In this study, a design is developed in which the probes are first incorporated in supported lipid bilayers formed around micron-sized particles (lipobeads), and the microbeads themselves are then arrayed on a surface by hydrodynamic capture in a microfluidic obstacle course of traps. Read More
    Microfluidic single-cell cultivation chip with controllable immobilization and selective release of yeast cells.
    Lab Chip 2012 Mar 22;12(5):906-15. Epub 2011 Dec 22.
    ETH Zurich, Department of Biosystems Science and Engineering (D-BSSE), Bio Engineering Laboratory (BEL), Basel, Switzerland.
    We present a microfluidic cell-culture chip that enables trapping, cultivation and release of selected individual cells. The chip is fabricated by a simple hybrid glass-SU-8-PDMS approach, which produces a completely transparent microfluidic system amenable to optical inspection. Single cells are trapped in a microfluidic channel using mild suction at defined cell immobilization orifices, where they are cultivated under controlled environmental conditions. Read More
    A microfluidic-based hydrodynamic trap: design and implementation.
    Lab Chip 2011 May 8;11(10):1786-94. Epub 2011 Apr 8.
    Department of Chemical and Biomolecular Engineering, University of Illinois Urbana-Champaign, 600 S. Mathews Ave., Urbana, IL, USA.
    We report an integrated microfluidic device for fine-scale manipulation and confinement of micro- and nanoscale particles in free-solution. Using this device, single particles are trapped in a stagnation point flow at the junction of two intersecting microchannels. The hydrodynamic trap is based on active flow control at a fluid stagnation point using an integrated on-chip valve in a monolithic PDMS-based microfluidic device. Read More