Array-based capture, distribution, counting and multiplexed assaying of beads on a centrifugal microfluidic platform.

Authors:
Robert Burger
Robert Burger
University of California
United States
Gregor Kijanka
Gregor Kijanka
Biomedical Diagnostics Institute
Ireland
Victor Akujobi
Victor Akujobi
Dublin City University
Ireland
Patrick Abgrall
Patrick Abgrall
Nanyang Technological University
Jiaxing | China
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Fraunhofer Project Centre at Dublin City University
Professor (Full)
microfluidics, Lab-on-a-Chip, hydrodynanmics, business development, project management, organisational leadership
Glasnevin, Dublin 9 | Ireland

Lab Chip 2012 Apr 15;12(7):1289-95. Epub 2012 Feb 15.

Biomedical Diagnostics Institute, National Centre for Sensor Research, School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.

We present a novel centrifugal microfluidic platform for the highly efficient manipulation and analysis of particles for applications in bead-based assays. The platform uses an array of geometrical V-cup barriers to trap particles using stopped-flow sedimentation under highly reproducible hydrodynamic conditions. The impact parameters governing the occupancy distribution and capture efficiency of the arrayed traps are investigated. The unique, nearly 100% capture efficiency paired with the capability to establish sharply peaked, single occupancy distributions enables a novel, digital readout mode for color-multiplexed, particle-based assays with low-complexity instrumentation. The presented technology marks an essential step towards a versatile platform for the integration of bead- and cell-based biological assays.

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http://dx.doi.org/10.1039/c2lc21170jDOI Listing

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April 2012
34 Reads
11 PubMed Central Citations(source)

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