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Ecotoxicology 2021 Apr 15. Epub 2021 Apr 15.

Department of General and Applied Biology, Institute of Biosciences, São Paulo State University (UNESP), Av. 24-A, 1515, CEP: 13506-900, Rio Claro, São Paulo, Brazil.

Vinasse sugarcane is a valuable byproduct of the ethanol production process, presenting a perspective of volume increase with the development of second generation ethanol (2GE). However, this byproduct needs new methods of treatment and management for sustainability. Besides that, 2GE vinasse can be associated with some compounds (such as furan derivatives, phenolic compounds and organic acids), depending on the process used to solubilize hemicellulose, which could compromise vinasse destination or utilization. Read More

Biotechnol Appl Biochem 2021 Apr 15. Epub 2021 Apr 15.

Enzymology and Fermentation Technology Laboratory, Health Department, State University of Feira de Santana, Feira de Santana, Bahia, Brazil.

β-glucosidases (BGLs) belong to the group of enzymes of cellulases and acts in the last stage of cellulose degradation, releasing glucose molecules, eliminating the inhibitory effect of cellobiose. This study focused on the production, characterization, and application of β-glucosidase from Moniliophthora perniciosa in the hydrolysis of pre-treated sugarcane bagasse (3% NaOH + 6% Na SO ), with varying enzymatic loads and reaction times. The enzyme showed an optimum pH of 4. Read More

Biophys J 2021 Apr 5. Epub 2021 Apr 5.

Microorganisms and General Biochemistry Laboratory, Institute of Bioscience (INBio), Federal University of Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brazil.

Understanding the aspects that contribute to improving proteins' biochemical properties is of high relevance for protein engineering. Properties such as the catalytic rate, thermal stability, and thermal resistance are crucial for applying enzymes in the industry. Different interactions can influence those biochemical properties of an enzyme. Read More

Microorganisms 2021 Mar 5;9(3). Epub 2021 Mar 5.

Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto 14049-900, Brazil.

The lignocellulosic biomass comprises three main components: cellulose, hemicellulose, and lignin. Degradation and conversion of these three components are attractive to biotechnology. This study aimed to prospect fungal lignocellulolytic enzymes with potential industrial applications, produced through a temporal analysis using and seeds as carbon sources. Read More

Waste Manag 2021 Mar 27;126:274-282. Epub 2021 Mar 27.

Laboratório de Química Orgânica, Departamento de Química, Universidade Federal do Espírito Santo (UFES), Avenida Fernando Ferrari, 514, Goiabeiras, Vitória, ES 29075-910, Brazil. Electronic address:

Levoglucosan (LGA) is a promising chemical platform derived from the pyrolysis of biomass that offers access to a variety of value-added products. We report an efficient route to produce LGA via the pretreatment of biomass with niobium compounds (oxalate, chloride and oxide) followed by fast pyrolysis coupled with gas chromatography-mass spectrometry (Py-GC-MS) at temperatures of 350-600 °C. Catalytic pretreatment reduces the quantity of lignin in the biomass, concentrates the cellulose and enhance LGA formation during fast pyrolysis. Read More