P-element homing is facilitated by engrailed polycomb-group response elements in Drosophila melanogaster.

PLoS One 2012 19;7(1):e30437. Epub 2012 Jan 19.

Program in Genomics of Differentiation, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America.

P-element vectors are commonly used to make transgenic Drosophila and generally insert in the genome in a nonselective manner. However, when specific fragments of regulatory DNA from a few Drosophila genes are incorporated into P-transposons, they cause the vectors to be inserted near the gene from which the DNA fragment was derived. This is called P-element homing. We mapped the minimal DNA fragment that could mediate homing to the engrailed/invected region of the genome. A 1.6 kb fragment of engrailed regulatory DNA that contains two Polycomb-group response elements (PREs) was sufficient for homing. We made flies that contain a 1.5 kb deletion of engrailed DNA (en(Δ1.5)) in situ, including the PREs and the majority of the fragment that mediates homing. Remarkably, homing still occurs onto the en(Δ1. 5) chromosome. In addition to homing to en, P[en] inserts near Polycomb group target genes at an increased frequency compared to P[EPgy2], a vector used to generate 18,214 insertions for the Drosophila gene disruption project. We suggest that homing is mediated by interactions between multiple proteins bound to the homing fragment and proteins bound to multiple areas of the engrailed/invected chromatin domain. Chromatin structure may also play a role in homing.

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0030437PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3261919PMC
June 2012
3 Reads

Publication Analysis

Top Keywords

homing
9
proteins bound
8
polycomb-group response
8
dna fragment
8
regulatory dna
8
response elements
8
p-element homing
8
fragment
5
dna
5
homing remarkably
4
majority fragment
4
remarkably homing
4
fragment mediates
4
mediates homing
4
occurs enΔ1
4
homing p[en]
4
p[en] inserts
4
addition homing
4
chromosome addition
4
pres majority
4

References

(Supplied by CrossRef)
Detection in situ of genomic regulatory elements in Drosophila.
CJ O'Kane et al.
Proc Natl Acad Sci USA 1987
A novel mechanism for P element homing in Drosophila.
E Taillebourg et al.
Proc Natl Acad Sci U S A 1999
P element homing to the Drosophila bithorax complex.
W Bender et al.
Development 2000
Rescue of Drosophila engrailed mutants with a highly divergent mosquito engrailed cDNA using a homing, enhancer-trapping transposon.
M Whiteley et al.
Development 1997

Similar Publications