J Biophotonics 2012 Mar 19;5(3):220-9. Epub 2011 Dec 19.
School of Physics and Astronomy, University of Nottingham, University Park, NG7 2RD, Nottingham, UK.
A method is presented for acquiring high-spatial-resolution spectral maps, in particular for Raman micro-spectroscopy (RMS), by selectively sampling the spatial features of interest and interpolating the results. This method achieves up to 30 times reduction in the sampling time compared to raster-scanning, the resulting images have excellent correlation with conventional histopathological staining, and are achieved with sufficient spectral signal-to-noise ratio to identify individual tissue structures. The benefits of this selective sampling method are not limited to tissue imaging however; it is expected that the method may be applied to other techniques which employ point-by-point mapping of large substrates.