Desulfurization of dibenzothiophene (DBT) by a novel strain Lysinibacillus sphaericus DMT-7 isolated from diesel contaminated soil.

J Environ Sci (China) 2011 ;23(6):975-82

Department of Biotechnology, Modern Institute of Technology (MIT), Dhalwala, Rishikesh 249201, Uttarakhand, India.

A new bacterial strain DMT-7 capable of selectively desulfurizing dibenzothiophene (DBT) was isolated from diesel contaminated soil. The DMT-7 was characterized and identified as Lysinibacillus sphaericus DMT-7 (NCBI GenBank Accession No. GQ496620) using 16S rDNA gene sequence analysis. The desulfurized product of DBT, 2-hydroxybiphenyl (2HBP), was identified and confirmed by high performance liquid chromatography analysis and gas chromatography-mass spectroscopy analysis respectively. The desulfurization kinetics revealed that DMT-7 started desulfurization of DBT into 2HBP after the lag phase of 24 hr, exponentially increasing the accumulation of 2HBP up to 15 days leading to approximately 60% desulfurization of the DBT. However, further growth resulted into DBT degradation. The induced culture of DMT-7 showed shorter lag phase of 6 hr and early onset of stationary phase within 10 days for desulfurization as compared to that of non-induced culture clearly indicating the inducibility of the desulfurization pathway of DMT-7. In addition, Lysinibacillus sphaericus DMT-7 also possess the ability to utilize broad range of substrates as sole source of sulfur such as benzothiophene, 3,4-benzo DBT, 4,6-dimethyl DBT, and 4,6-dibutyl DBT. Therefore, Lysinibacillus sphaericus DMT-7 could serve as model system for efficient biodesulfurization of diesel and petrol.

Download full-text PDF

Source
http://dx.doi.org/10.1016/s1001-0742(10)60504-9DOI Listing
January 2012
4 Reads
1 Citation
2.000 Impact Factor

Publication Analysis

Top Keywords

sphaericus dmt-7
16
lysinibacillus sphaericus
16
dbt
9
dmt-7
9
desulfurization dbt
8
diesel contaminated
8
lag phase
8
isolated diesel
8
contaminated soil
8
dibenzothiophene dbt
8
desulfurization
6
degradation induced
4
induced culture
4
dbt degradation
4
growth dbt
4
leading 60%
4
60% desulfurization
4
dbt growth
4
culture dmt-7
4
onset stationary
4

Similar Publications