Platelet adhesion and degranulation induce pro-survival and pro-angiogenic signalling in ovarian cancer cells.

Authors:
Karl Egan
Karl Egan
Royal College of Surgeons in Ireland
Ireland
Paul Smyth
Paul Smyth
Dartmouth Medical School
United States
Cathy Spillane
Cathy Spillane
Trinity College Dublin
Ireland
Cara Martin
Cara Martin
Trinity College Dublin
Ireland
Michael Gallagher
Michael Gallagher
William Beaumont Hospital
United States
Aoife Canney
Aoife Canney
St Vincent's University Hospital

PLoS One 2011 12;6(10):e26125. Epub 2011 Oct 12.

Molecular and Cellular Therapeutics, The Royal College of Surgeons in Ireland, Dublin, Ireland.

Thrombosis is common in ovarian cancer. However, the interaction of platelets with ovarian cancer cells has not been critically examined. To address this, we investigated platelet interactions in a range of ovarian cancer cell lines with different metastatic potentials [HIO-80, 59M, SK-OV-3, A2780, A2780cis]. Platelets adhered to ovarian cancer cells with the most significant adhesion to the 59M cell line. Ovarian cancer cells induced platelet activation [P-selectin expression] in a dose dependent manner, with the most significant activation seen in response to the 59M cell line. The platelet antagonists [cangrelor, MRS2179, and apyrase] inhibited 59M cell induced activation suggesting a P2Y12 and P2Y1 receptor mediated mechanism of platelet activation dependent on the release of ADP by 59M cells. A2780 and 59M cells potentiated PAR-1, PAR-4, and TxA2 receptor mediated platelet activation, but had no effect on ADP, epinephrine, or collagen induced activation. Analysis of gene expression changes in ovarian cancer cells following treatment with washed platelets or platelet releasate showed a subtle but valid upregulation of anti-apoptotic, anti-autophagy pro-angiogenic, pro-cell cycle and metabolic genes. Thus, ovarian cancer cells with different metastatic potential adhere and activate platelets differentially while both platelets and platelet releasate mediate pro-survival and pro-angiogenic signals in ovarian cancer cells.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0026125PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3192146PMC

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February 2012
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41 PubMed Central Citations(source)
3.23 Impact Factor

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References

(Supplied by CrossRef)
Ovarian cancer: epidemiology, biology, and prognostic factors.
CH Holschneider et al.
Semin Surg Oncol 2000
Occult tumor cells in bone marrow of patients with locoregionally restricted ovarian cancer predict early distant metastatic relapse.
S Braun et al.
J Clin Oncol 2001
Antimetastatic effects associated with platelet reduction.
GJ Gasic et al.
Proc Natl Acad Sci U S A 1968
P-selectin deficiency attenuates tumor growth and metastasis.
YJ Kim et al.
Proc Natl Acad Sci U S A 1998
Protection from tumor necrosis factor-mediated cytolysis by platelets.
C Philippe et al.
Am J Pathol 1993

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