Nanoparticle microinjection and Raman spectroscopy as tools for nanotoxicology studies.

Analyst 2011 Nov 30;136(21):4402-8. Epub 2011 Aug 30.

BioNEM Laboratory, Experimental and Clinical Medicine Department, University Magna Graecia of Catanzaro, 88100 Loc. Germaneto, Catanzaro, Italy.

Microinjection techniques and Raman spectroscopy have been combined to provide a new methodology to investigate the cytotoxic effects due to the interaction of nanomaterials with cells. In the present work, this novel technique has been used to investigate the effects of Ag and Fe(3)O(4) nanoparticles on Hela cells. The nanoparticles are microinjected inside the cells and these latter ones are probed by means of Raman spectroscopy after a short incubation time, in order to highlight the first and impulsive mechanisms developed by the cells to counteract the presence of the nanoparticles. The results put in evidence a different behaviour of the cells treated with nanoparticles in comparison with the control cells; these differences are supposed to be generated by an emerging oxidative stress due to the nanoparticles. The achieved results demonstrate the suitability of the proposed method as a new tool for nanotoxicity studies.

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http://dx.doi.org/10.1039/c1an15313gDOI Listing
November 2011
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