Integrated microfluidic array plate (iMAP) for cellular and molecular analysis.

Authors:
Ivan K Dimov
Ivan K Dimov
Dublin City University
Ireland
Gregor Kijanka
Gregor Kijanka
Biomedical Diagnostics Institute
Ireland
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Fraunhofer Project Centre at Dublin City University
Professor (Full)
microfluidics, Lab-on-a-Chip, hydrodynanmics, business development, project management, organisational leadership
Glasnevin, Dublin 9 | Ireland
Taewook Kang
Taewook Kang
Seoul National University
South Korea
Luke P Lee
Luke P Lee
University of California
United States

Lab Chip 2011 Aug 28;11(16):2701-10. Epub 2011 Jun 28.

Biomedical Diagnostics Institute, NCSR, Dublin City University, Glasnevin, Dublin, 9, Ireland.

Just as the Petri dish has been invaluable to the evolution of biomedical science in the last 100 years, microfluidic cell assay platforms have the potential to change significantly the way modern biology and clinical science are performed. However, an evolutionary process of creating an efficient microfluidic array for many different bioassays is necessary. Specifically for a complete view of a cell response it is essential to incorporate cytotoxic, protein and gene analysis on a single system. Here we present a novel cellular and molecular analysis platform, which allows access to gene expression, protein immunoassay, and cytotoxicity information in parallel. It is realized by an integrated microfluidic array plate (iMAP). The iMAP enables sample processing of cells, perfusion based cell culture, effective perturbation of biologic molecules or drugs, and simultaneous, real-time optical analysis for different bioassays. The key features of the iMAP design are the interface of on-board gravity driven flow, the open access input fluid exchange and the highly efficient sedimentation based cell capture mechanism (∼100% capture rates). The operation of the device is straightforward (tube and pump free) and capable of handling dilute samples (5-cells per experiment), low reagent volumes (50 nL per reaction), and performing single cell protein and gene expression measurements. We believe that the unique low cell number and triple analysis capabilities of the iMAP platform can enable novel dynamic studies of scarce cells.

Download full-text PDF

Source
http://dx.doi.org/10.1039/c1lc20105kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143330PMC
August 2011
32 Reads
12 Citations

Publication Analysis

Top Keywords

microfluidic array
12
cellular molecular
8
protein gene
8
molecular analysis
8
gene expression
8
based cell
8
plate imap
8
integrated microfluidic
8
array plate
8
cell
6
imap
5
analysis
5
design interface
4
features imap
4
imap design
4
bioassays key
4
optical analysis
4
analysis bioassays
4
interface on-board
4
key features
4

Altmetric Statistics

References

(Supplied by CrossRef)
Article in Anal. Chem.
Figeys et al.
Anal. Chem. 2000
Article in Anal. Chem.
Dittrich et al.
Anal. Chem. 2006
Article in Anal. Chem.
Khandurina et al.
Anal. Chem. 2000
Article in Lab Chip
Kamei et al.
Lab Chip 2010
Article in Nature
Nagrath et al.
Nature 2007
Article in Lab Chip
Sims et al.
Lab Chip 2007
Article in Lab Chip
Keenan et al.
Lab Chip 2008
Article in Annu. Rev. Biomed. Eng.
Folch et al.
Annu. Rev. Biomed. Eng. 2000
Article in Lab Chip
Kim et al.
Lab Chip 2007
Article in Lab Chip
Yu et al.
Lab Chip 2007
Article in Lab Chip
Chung et al.
Lab Chip 2005

Similar Publications