Integrated microfluidic array plate (iMAP) for cellular and molecular analysis.

Ivan K Dimov
Ivan K Dimov
Dublin City University
Gregor Kijanka
Gregor Kijanka
Biomedical Diagnostics Institute
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Fraunhofer Project Centre at Dublin City University
Professor (Full)
microfluidics, Lab-on-a-Chip, hydrodynanmics, business development, project management, organisational leadership
Glasnevin, Dublin 9 | Ireland
Taewook Kang
Taewook Kang
Seoul National University
South Korea
Luke P Lee
Luke P Lee
University of California
United States

Lab Chip 2011 Aug 28;11(16):2701-10. Epub 2011 Jun 28.

Biomedical Diagnostics Institute, NCSR, Dublin City University, Glasnevin, Dublin, 9, Ireland.

Just as the Petri dish has been invaluable to the evolution of biomedical science in the last 100 years, microfluidic cell assay platforms have the potential to change significantly the way modern biology and clinical science are performed. However, an evolutionary process of creating an efficient microfluidic array for many different bioassays is necessary. Specifically for a complete view of a cell response it is essential to incorporate cytotoxic, protein and gene analysis on a single system. Here we present a novel cellular and molecular analysis platform, which allows access to gene expression, protein immunoassay, and cytotoxicity information in parallel. It is realized by an integrated microfluidic array plate (iMAP). The iMAP enables sample processing of cells, perfusion based cell culture, effective perturbation of biologic molecules or drugs, and simultaneous, real-time optical analysis for different bioassays. The key features of the iMAP design are the interface of on-board gravity driven flow, the open access input fluid exchange and the highly efficient sedimentation based cell capture mechanism (∼100% capture rates). The operation of the device is straightforward (tube and pump free) and capable of handling dilute samples (5-cells per experiment), low reagent volumes (50 nL per reaction), and performing single cell protein and gene expression measurements. We believe that the unique low cell number and triple analysis capabilities of the iMAP platform can enable novel dynamic studies of scarce cells.
PDF Download - Full Text Link
( Please be advised that this article is hosted on an external website not affiliated with
Source Status ListingPossible
August 2011
8 Reads
11 PubMed Central Citations(source)

Similar Publications

Pressure-driven perfusion culture microchamber array for a parallel drug cytotoxicity assay.

Biotechnol Bioeng 2008 Aug;100(6):1156-65

Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology, Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.

This article reports a pressure-driven perfusion culture chip developed for parallel drug cytotoxicity assay. The device is composed of an 8 x 5 array of cell culture microchambers with independent perfusion microchannels. It is equipped with a simple interface for convenient access by a micropipette and connection to an external pressure source, which enables easy operation without special training. Read More

View Article
August 2008

Continuous perfusion microfluidic cell culture array for high-throughput cell-based assays.

Biotechnol Bioeng 2005 Jan;89(1):1-8

Berkeley Sensor & Actuator Center, Department of Bioengineering, University of California, 485 Evans Hall, Berkeley, CA 94720, USA.

We present for the first time a microfluidic cell culture array for long-term cellular monitoring. The 10 x 10 array could potentially assay 100 different cell-based experiments in parallel. The device was designed to integrate the processes used in typical cell culture experiments on a single self-contained microfluidic system. Read More

View Article
January 2005

A programmable microfluidic cell array for combinatorial drug screening.

Lab Chip 2012 Apr 28;12(10):1813-22. Epub 2012 Mar 28.

Department of Chemical Engineering, Texas A&M University, College Station, TX 77843-3122, USA.

We describe the development of a fully automatic and programmable microfluidic cell culture array that integrates on-chip generation of drug concentrations and pair-wise combinations with parallel culture of cells for drug candidate screening applications. The device has 64 individually addressable cell culture chambers in which cells can be cultured and exposed either sequentially or simultaneously to 64 pair-wise concentration combinations of two drugs. For sequential exposure, a simple microfluidic diffusive mixer is used to generate different concentrations of drugs from two inputs. Read More

View Article
April 2012

Microfluidic serial dilution cell-based assay for analyzing drug dose response over a wide concentration range.

Anal Chem 2010 Oct;82(19):8278-82

Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Central fifth, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.

In this paper we report a perfusion culture microchamber array chip with a serial dilution microfluidic network for analyzing drug dose response over a concentration range spanning 6 orders of magnitude, which is required for practical drug discovery applications. The microchamber array chip was equipped with a pressure-driven interface, in which medium and drug solution were added with a micropipet and delivered into the microfluidic network by pneumatic pressure. We demonstrated that the microchamber array chip could be used to estimate the 50% growth inhibitory concentration using the model anticancer drug paclitaxel and the model cancer cell line HeLa. Read More

View Article
October 2010