Lab Chip 2011 Aug 28;11(16):2701-10. Epub 2011 Jun 28.
Biomedical Diagnostics Institute, NCSR, Dublin City University, Glasnevin, Dublin, 9, Ireland.
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Biotechnol Bioeng 2008 Aug;100(6):1156-65
Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology, Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
This article reports a pressure-driven perfusion culture chip developed for parallel drug cytotoxicity assay. The device is composed of an 8 x 5 array of cell culture microchambers with independent perfusion microchannels. It is equipped with a simple interface for convenient access by a micropipette and connection to an external pressure source, which enables easy operation without special training. Read More
Biotechnol Bioeng 2005 Jan;89(1):1-8
Berkeley Sensor & Actuator Center, Department of Bioengineering, University of California, 485 Evans Hall, Berkeley, CA 94720, USA.
We present for the first time a microfluidic cell culture array for long-term cellular monitoring. The 10 x 10 array could potentially assay 100 different cell-based experiments in parallel. The device was designed to integrate the processes used in typical cell culture experiments on a single self-contained microfluidic system. Read More
Lab Chip 2012 Apr 28;12(10):1813-22. Epub 2012 Mar 28.
Department of Chemical Engineering, Texas A&M University, College Station, TX 77843-3122, USA.
We describe the development of a fully automatic and programmable microfluidic cell culture array that integrates on-chip generation of drug concentrations and pair-wise combinations with parallel culture of cells for drug candidate screening applications. The device has 64 individually addressable cell culture chambers in which cells can be cultured and exposed either sequentially or simultaneously to 64 pair-wise concentration combinations of two drugs. For sequential exposure, a simple microfluidic diffusive mixer is used to generate different concentrations of drugs from two inputs. Read More
Anal Chem 2010 Oct;82(19):8278-82
Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Central fifth, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
In this paper we report a perfusion culture microchamber array chip with a serial dilution microfluidic network for analyzing drug dose response over a concentration range spanning 6 orders of magnitude, which is required for practical drug discovery applications. The microchamber array chip was equipped with a pressure-driven interface, in which medium and drug solution were added with a micropipet and delivered into the microfluidic network by pneumatic pressure. We demonstrated that the microchamber array chip could be used to estimate the 50% growth inhibitory concentration using the model anticancer drug paclitaxel and the model cancer cell line HeLa. Read More