Identification, sequencing and molecular analysis of Chp4, a novel chlamydiaphage of Chlamydophila abortus belonging to the family Microviridae.

J Gen Virol 2011 Jul 30;92(Pt 7):1733-7. Epub 2011 Mar 30.

Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK.

Members of the family Microviridae have been identified in a number of chlamydial species infecting humans (phage CPAR39 in Chlamydophila pneumoniae), other mammals (φCPG1 in Chlamydophila caviae, Chp2 in Chlamydophila abortus and Chp3 in Chlamydophila pecorum) and birds (Chp1 in Chlamydophila psittaci). This study describes the identification and genome sequencing of Chp4, an icosahedral, 4530 bp, ssDNA phage in C. abortus. Chp4 is predicted to contain eight ORFs, six of which could be assigned putative functions based on sequence similarity to characterized bacteriophage. Gene order and content were highly conserved amongst chlamydiaphage, with the highest sequence variability occurring in the IN5 and INS variable regions of the VP1 major coat protein, which has been associated with host cell recognition and binding. Phylogenetic analysis of VP1 indicated that Chp4 is a member of the Chlamydiamicrovirus, and is most closely related to phage φCPG1 and CPAR39.

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http://dx.doi.org/10.1099/vir.0.031583-0DOI Listing
July 2011
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References

(Supplied by CrossRef)
Article in Journal of Biological Chemistry
Aoyama et al.
Journal of Biological Chemistry 1983
Article in Journal of molecular biology
Bernal et al.
Journal of molecular biology 2004
Article in Journal of Bacteriology
Brentlinger et al.
Journal of Bacteriology 2002
Article in Structure (London)
Chipman et al.
Structure (London) 1998
Article in Nucleic Acids Research
Edgar et al.
Nucleic Acids Research 2004
Article in Journal of Biological Chemistry
Eisenberg et al.
Journal of Biological Chemistry 1979
Article in Journal of Bacteriology
Everson et al.
Journal of Bacteriology 2002
Article in Journal of Bacteriology
Everson et al.
Journal of Bacteriology 2003
Article in Virus genes
Garner et al.
Virus genes 2004
Article in Journal of comparative pathology
Graham et al.
Journal of comparative pathology 1995
Article in Microbes and infection / Institut Pasteur
Hsia et al.
Microbes and infection / Institut Pasteur 2000

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