J Vis Exp 2011 Feb 15(48). Epub 2011 Feb 15.
Swedish Medical Nanoscience Center, Department of Neuroscience, Karolinska Institutet.
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J Biol Rhythms 2014 Apr;29(2):110-8
MRC Laboratory of Molecular Biology, Cambridge, UK.
The suprachiasmatic nucleus (SCN) of the hypothalamus is the principal circadian pacemaker in mammals, coordinating daily metabolic and physiological rhythms with the cycle of sleep and wakefulness. SCN neurons define circadian time via an auto-regulatory feedback loop in which the activation of Period (Per) and Cryptochrome genes is periodically suppressed by their own protein products. Casein kinase 1 (CK1) enzymes have a critical role in circadian pacemaking because they phosphorylate PER proteins and thereby direct their proteasomal degradation. Read More
J Biol Rhythms 2015 Oct 6;30(5):396-407. Epub 2015 Jul 6.
Department of Biology, Washington University, St. Louis, MO, USA.
Neurons in the suprachiasmatic nucleus (SCN), the master circadian pacemaker in mammals, display daily rhythms in electrical activity with more depolarized resting potentials and higher firing rates during the day than at night. Although these daily variations in the electrical properties of SCN neurons are required for circadian rhythms in physiology and behavior, the mechanisms linking changes in neuronal excitability to the molecular clock are not known. Recently, we reported that mice deficient for either Kcna4 (Kv1. Read More
eNeuro 2017 Jul-Aug;4(4). Epub 2017 Aug 18.
Department of Psychiatry and Center for Circadian Biology, University of California, San Diego, La Jolla, CA 92093-0603.
Circadian rhythms of mammalian physiology and behavior are coordinated by the suprachiasmatic nucleus (SCN) in the hypothalamus. Within SCN neurons, various aspects of cell physiology exhibit circadian oscillations, including circadian clock gene expression, levels of intracellular Ca ([Ca]), and neuronal firing rate. [Ca] oscillates in SCN neurons even in the absence of neuronal firing. Read More
Eur J Neurosci 2015 Dec;42(12):3128-37
Department of Chronomedicine, Hokkaido University Graduate School of Medicine, North 15, West 7, Kita-ku, Sapporo, 060-8638, Japan.
The temporal order of physiology and behaviour in mammals is regulated by the coordination of the master circadian clock in the suprachiasmatic nucleus (SCN) and peripheral clocks in various tissues outside the SCN. Because the circadian oscillator(s) in the olfactory bulb (OB) is regarded as SCN independent, we examined the relationship between the SCN master clock and the circadian clock in the OB. We also examined the role of vasoactive intestinal peptide receptor 2 in the circadian organization of the OB. Read More