Get 20% Off Journals at

Impact of high-throughput screening in biomedical research.

Nat Rev Drug Discov 2011 03;10(3):188-95

Department of Sample Management Technologies, GlaxoSmithKline, Mail Stop UP 12-200, 1250S Collegeville Rd, Collegeville, Pennsylvania 19426, USA.

High-throughput screening (HTS) has been postulated in several quarters to be a contributory factor to the decline in productivity in the pharmaceutical industry. Moreover, it has been blamed for stifling the creativity that drug discovery demands. In this article, we aim to dispel these myths and present the case for the use of HTS as part of a proven scientific tool kit, the wider use of which is essential for the discovery of new chemotypes.

Download full-text PDF

Source Listing
March 2011
Get 20% Off Journals at

Similar Publications

Efficient influence of ssDNA virus PCV2 replication by CRISPR/Cas9 targeting of the viral genome.

Mol Immunol 2021 Feb 22;133:63-66. Epub 2021 Feb 22.

Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, 250100, China; Qingdao Agricultural University, Qingdao, 266000, China; Shandong Normal University, Jinan, 250014, China. Electronic address:

Porcine circovirus type 2 (PCV2), a ubiquitous pathogen that primary cause of postweaning multisystemic wasting syndrome (PMWS), had caused significant morbidity and mortality in swine populations with huge economic losses in the worldwide swine industry. Currently, looking for effective antiviral drugs for PCV2 infection remains an important works. In our study, CRISPR/Cas9 system was used to further detected the key sites of PCV2 replication. Read More

View Article and Full-Text PDF
February 2021

Optimal secretion of thermostable Beta-glucosidase in Bacillus subtilis by signal peptide optimization.

Protein Expr Purif 2021 Feb 22:105843. Epub 2021 Feb 22.

DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology (formerly UDCT), Nathalal Parikh Marg, Matunga, Mumbai, 400019, India. Electronic address:

Commercial applications of β-glucosidase (BGL) demands its purity and availability on a large scale. In the present study, we aim to optimize the expression and secretion of a thermostable BGL from Pyrococcus furiosus (PfuBGL) in B. subtilis strain RIK1285. Read More

View Article and Full-Text PDF
February 2021

Molecular basis for the interaction of cellular retinol-binding protein 2 (CRBP2) with non-retinoid ligands.

J Lipid Res 2021 Feb 22:100054. Epub 2021 Feb 22.

The Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH; The Cleveland Center for Membrane and Structural Biology, School of Medicine, Case Western Reserve University, Cleveland, OH. Electronic address:

Present in the small intestine, cellular retinol-binding protein 2 (CRBP2) plays an important role in the uptake, transport, and metabolism of dietary retinoids. However, the recent discovery of the interactions of CRBP2 with 2-arachidonoylglycerol (2-AG) and other monoacylglycerols (MAGs) suggests the broader involvement of this protein in lipid metabolism and signaling. To better understand the physiological role of CRBP2, we determined its protein-lipid interactome using a fluorescence-based retinol replacement assay adapted for a high-throughput screening format. Read More

View Article and Full-Text PDF
February 2021

Re-defining synthetic lethality by phenotypic profiling for precision oncology.

Cell Chem Biol 2021 Feb 16. Epub 2021 Feb 16.

Institute for Molecular Medicine Finland (FIMM), HiLIFE, University of Helsinki, Helsinki, Finland; Institute for Cancer Research, Department of Cancer Genetics, Oslo University Hospital, Oslo, Norway; Centre for Biostatistics and Epidemiology (OCBE), Faculty of Medicine, University of Oslo, Oslo, Norway. Electronic address:

High-throughput functional and genomic screening techniques provide systematic means for phenotypic discovery. Using synthetic lethality (SL) as a paradigm for anticancer drug and target discovery, we describe how these screening technologies may offer new possibilities to identify therapeutically relevant and selective SL interactions by addressing some of the challenges that have made robust discovery of SL candidates difficult. We further introduce an extended concept of SL interaction, in which a simultaneous perturbation of two or more cellular components reduces cell viability more than expected by their individual effects, which we feel is highly befitting for anticancer applications. Read More

View Article and Full-Text PDF
February 2021

Robust Multichannel Encoding for Highly Multiplexed Quantitative PCR.

Anal Chem 2021 Feb 25. Epub 2021 Feb 25.

ChromaCode Inc., 2330 Faraday Ave Suite 100, Carlsbad, California 92008, United States.

The gold standard of molecular pathogen detection is the quantitative polymerase chain reaction (qPCR). Modern qPCR instruments are capable of detecting 4-6 analytes in a single sample: one per optical detection channel. However, many clinical applications require multiplexing beyond this traditional single-well capacity, including the task of simultaneously testing for SARS-CoV-2 and other respiratory pathogens. Read More

View Article and Full-Text PDF
February 2021