Circulating tumour cells lacking cytokeratin in breast cancer: the importance of being mesenchymal.

J Cell Mol Med 2011 May;15(5):1066-70

Department of Molecular Medicine, Sapienza University, Viale Regina Elena, Rome, Italy.

Circulating tumour cells (CTCs) are independent predictor of prognosis in metastatic breast cancer. Nevertheless, in one third of patients, circulating tumour cells are undetected by conventional methods. Aim of the study was to assess the prognostic value of circulating tumour cells expressing mesenchymal markers in metastatic breast cancer patients. We isolated CTC from blood of 55 metastatic breast cancer patients. CTC were characterized for cytokeratins and markers of epithelial mesenchymal transition. The gain of mesenchymal markers in CTC was correlated to prognosis of patients in a follow-up of 24 months. The presence of mesenchymal markers on CTC more accurately predicted worse prognosis than the expression of cytokeratins alone. Because of the frequent loss of epithelial antigens by CTC, assays targeting epithelial antigens may miss the most invasive cell population. Thus, there is an urgent need to improve detection methods to identify CTC which undergone epithelial mesenchymal transition program.

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1582-4934.2011.01285.xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822619PMC
May 2011
5 Reads

Publication Analysis

Top Keywords

tumour cells
16
breast cancer
16
circulating tumour
16
mesenchymal markers
12
metastatic breast
12
epithelial mesenchymal
8
mesenchymal transition
8
markers ctc
8
cancer patients
8
epithelial antigens
8
mesenchymal
6
ctc
6
ctc correlated
4
correlated prognosis
4
presence mesenchymal
4
follow-up months
4
patients follow-up
4
months presence
4
prognosis patients
4
markers epithelial
4

Altmetric Statistics

References

(Supplied by CrossRef)
Cytokeratin and vimentin expression in breast cancer
Vora et al.
Int J Biol Markers. 2009
Characterization of metastatic breast cancer patients with non-detectable circulating tumor cells
Mego et al.
Int J Cancer 2010
Immunobead-PCR: a technique for the detection of circulating tumor cells using immunomagnetic beads and the polymerase chain reaction
Hardingham et al.
Cancer Res. 1993

Similar Publications