From EosFP to mIrisFP: structure-based development of advanced photoactivatable marker proteins of the GFP-family.

Authors:
Dr. Virgile Adam, PhD
Dr. Virgile Adam, PhD
CNRS / Institute for Structural Biology (IBS)
Research scientist
single molecule fluorescence microscopy, fluorescent protein engineering, X-ray crystallography
Grenoble | France

J Biophotonics 2011 Jun 14;4(6):377-90. Epub 2011 Feb 14.

National Oceanography Centre, University of Southampton, Southampton SO143ZH, UK.

Fluorescent proteins from the GFP family have become indispensable imaging tools in life sciences research. In recent years, a wide variety of these proteins were discovered in non-bioluminescent anthozoa. Some of them feature exciting new properties, including the possibility to change their fluorescence quantum yield and/or color by irradiating with light of specific wavelengths. These photoactivatable fluorescent proteins enable many interesting applications including pulse-chase experiments and super-resolution imaging. In this review, we discuss the development of advanced variants, using a structure-function based, molecular biophysics approach, of the photoactivatable fluorescent protein EosFP, which can be photoconverted from green to red fluorescence by ~400 nm light. A variety of applications are presented that demonstrate the versatility of these marker proteins in live-cell imaging.

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http://dx.doi.org/10.1002/jbio.201000122DOI Listing
June 2011
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References

(Supplied by CrossRef)

Nienhaus et al.
Angew. Chem. Int. Ed. Engl. 2008

Shimomura et al.
J. Cell. Comp. Physiol. 1962

Chalfie et al.
Science 1994

Prasher et al.
Gene 1992

Matz et al.
Nat. Biotechnol. 1999

Wiedenmann et al.
P. Natl. Acad. Sci. USA 2000

Nienhaus et al.
Chem. Phys. Chem. 2009

Wiedenmann et al.
Expert Rev. Proteomics 2006

Patterson et al.
Science 2002

Habuchi et al.
Proc. Natl. Acad. Sci. USA 2005

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