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Sci Rep 2021 Apr 7;11(1):8015. Epub 2021 Apr 7.

Cell Surface Signalling Laboratory, Wellcome Sanger Institute, Cambridge, UK.

Nanomaterials (Basel) 2021 Mar 8;11(3). Epub 2021 Mar 8.

Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618 Tallinn, Estonia.

In the current study, the antibacterial activity of positively and negatively charged spherical hematite (-FeO) nanoparticles (NPs) with primary size of 45 and 70 nm was evaluated against clinically relevant bacteria (gram-negative) and (gram-positive) as well as against naturally bioluminescent bacteria (an ecotoxicological model organism). -FeO NPs were synthesized using a simple green hydrothermal method and the surface charge was altered via citrate coating. To minimize the interference of testing environment with NP's physic-chemical properties, and were exposed to NPs in deionized water for 30 min and 24 h, covering concentrations from 1 to 1000 mg/L. Read More

Sci Rep 2021 Apr 1;11(1):7424. Epub 2021 Apr 1.

Institute of Soil, Water and Environmental Sciences, Agricultural Research Organization, The Volcani Center, P.O. Box 15159, 7505101, Rishon LeZion, Israel.

Microplastics are ubiquitous in aquatic systems and break down into submicron particles that can interact with aquatic toxic chemicals. These interactions may affect the detection of toxicants when using bacteria as a biomonitoring tool. This study examined the effects of model polystyrene (PS)-based submicron particles on the detection of aqueous geno- and cytotoxicity by genetically modified bioluminescent (GMB) bacteria. Read More

J Microbiol Methods 2021 Mar 23:106209. Epub 2021 Mar 23.

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China. Electronic address:

Coupling loop-mediated isothermal amplification (LAMP) with a bioluminescent assay in real-time (LAMP-BART) is a strategy that can be readily leveraged to detect bacteria in particular samples of interest without the need for costly or complicated equipments. However, this approach exhibits poor sensitivity, and it additionally amplifies all target DNA including that derived from non-viable cells. Herein, we sought to overcome these traditional pyrophosphate bioluminescent assay limitations by utilizing 2-deoxyadenosine-5-(α-thio) -triphosphate (dATPαS) in place of dATP when conducting LAMP, thereby markedly reducing and stabilizing overall background signal levels, resulting in a detection limit of 3 CFU/μL. Read More

Sci Rep 2021 Mar 19;11(1):6431. Epub 2021 Mar 19.

Department of General Zoology and Neurobiology, Institute of Biology and Biotechnology, Ruhr-University Bochum, 44780, Bochum, Germany.

The schooling flashlight fish Anomalops katoptron can be found at dark nights at the water surface in the Indo-Pacific. Schools are characterized by bioluminescent blink patterns of sub-ocular light organs densely-packed with bioluminescent, symbiotic bacteria. Here we analyzed how blink patterns of A. Read More