The viral latency-associated nuclear antigen augments the B-cell response to antigen in vivo.

Authors:
Sang-Hoon Sin
Sang-Hoon Sin
Dept. Microbiology & Immunology / University of North Carolina at Chapel Hill
Research Assistant Professor
KSHV
Chapel Hill, NC | United States

J Virol 2010 Oct 4;84(20):10653-60. Epub 2010 Aug 4.

Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, Center for AIDS Research, University of North Carolina at Chapel Hill, CB#7290, 715 Mary Ellen Jones Bldg, Chapel Hill, NC 27599-7290, USA.

Gammaherpesviruses, including Kaposi sarcoma-associated herpesvirus (KSHV), establish latency in B cells. We hypothesized that the KSHV latency-associated nuclear antigen (LANA/orf73) provides a selective advantage to infected B cells by driving proliferation in response to antigen. To test this, we used LANA B-cell transgenic mice. Eight days after immunization with antigen without adjuvant, LANA mice had significantly more activated germinal center (GC) B cells (CD19(+) PNA(+) CD71(+)) than controls. This was dependent upon B-cell receptor since LANA did not restore the GC defect of CD19 knockout mice. However, LANA was able to restore the marginal zone defect in CD19 knockout mice.

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Source
http://dx.doi.org/10.1128/JVI.00848-10DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2950586PMC
October 2010
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