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    Chemical imaging with desorption electrospray ionization mass spectrometry.
    Methods Mol Biol 2010 ;656:231-41
    Organic and Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.
    Desorption electrospray ionization mass spectrometry (DESI-MS) is a relatively new ambient surface analysis method. This technique requires little or no sample preparation prior to the actual analysis. Along with other application areas, DESI-MS is used to obtain chemical images of different biomolecules in biological tissue sections. In the case of biological tissue analysis, only preparation of the tissue slices is necessary. Furthermore, the method does not require the use of an ionization matrix preventing the redistribution of analytes prior to the analysis. Chemical images are obtained by monitoring the mass spectrometric signals of compounds while moving the sample relative to the DESI sprayer.

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    Nat Protoc 2008 ;3(3):517-24
    Department of Chemistry, Purdue University, West Lafayette, Indiana, USA.
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    Faraday Discuss 2011 ;149:247-67; discussion 333-56
    Department of Chemistry and Center for Analytical Instrumentation Development, Purdue University, West Lafayette, IN 47907, USA.
    The state-of-the-art in two new ambient ionization methods for mass spectrometry, desorption electrospray ionization (DESI) and paper spray (PS), is described and their utility is illustrated with new studies on tissue imaging and biofluid analysis. DESI is an ambient ionization method that can be performed on untreated histological sections of biological tissue in the open lab environment to image lipids, fatty acids, hormones and other compounds. Paper spray is performed in the open lab too; it involves electrospraying dry blood spots or biofluid deposits from a porous medium. Read More
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    Organic and Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6131, USA.
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    Methods Mol Biol 2015 ;1203:107-16
    Department of Chemistry, College of Science, National Sun Yat-Sen University, No. 70, Lienhai Rd., Kaohsiung, 80424, Taiwan.
    The use of an ambient ionization mass spectrometry technique known as electrospray laser desorption ionization mass spectrometry (ELDI/MS) for molecular imaging is described in this section. The technique requires little or no sample pretreatment and the application of matrix on sample surfaces is unnecessary. In addition, the technique is highly suitable for the analysis of hard and thick tissues compared to other molecular imaging methods because it does not require production of thin tissue slices via microtomes, which greatly simplifies the overall sample preparation procedure and prevents the redistribution of analytes during matrix desorption. Read More