Methods Mol Biol 2010 ;656:231-41
Organic and Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.
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Nat Protoc 2008 ;3(3):517-24
Department of Chemistry, Purdue University, West Lafayette, Indiana, USA.
Desorption electrospray ionization (DESI) allows the direct analysis of ordinary objects or pre-processed samples under ambient conditions. Among other applications, DESI is used to identify and record spatial distributions of lipids and drug molecules in biological tissue sections. This technique does not require sample preparation other than production of microtome tissue slices and does not involve the use of ionization matrices. Read More
Faraday Discuss 2011 ;149:247-67; discussion 333-56
Department of Chemistry and Center for Analytical Instrumentation Development, Purdue University, West Lafayette, IN 47907, USA.
The state-of-the-art in two new ambient ionization methods for mass spectrometry, desorption electrospray ionization (DESI) and paper spray (PS), is described and their utility is illustrated with new studies on tissue imaging and biofluid analysis. DESI is an ambient ionization method that can be performed on untreated histological sections of biological tissue in the open lab environment to image lipids, fatty acids, hormones and other compounds. Paper spray is performed in the open lab too; it involves electrospraying dry blood spots or biofluid deposits from a porous medium. Read More
Anal Chem 2008 Jul 16;80(13):5168-77. Epub 2008 May 16.
Organic and Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6131, USA.
Desorption electrospray ionization tandem mass spectrometry (DESI-MS/MS) and whole-body autoradiography (WBA) were used for chemical imaging of whole-body thin tissue sections of mice intravenously dosed with propranolol (7.5 mg/kg). DESI-MS/MS imaging utilized selected reaction monitoring detection performed on an AB/MDS SCIEX 4000 QTRAP mass spectrometer equipped with a prototype extended length particle discriminator interface. Read More
Methods Mol Biol 2015 ;1203:107-16
Department of Chemistry, College of Science, National Sun Yat-Sen University, No. 70, Lienhai Rd., Kaohsiung, 80424, Taiwan.
The use of an ambient ionization mass spectrometry technique known as electrospray laser desorption ionization mass spectrometry (ELDI/MS) for molecular imaging is described in this section. The technique requires little or no sample pretreatment and the application of matrix on sample surfaces is unnecessary. In addition, the technique is highly suitable for the analysis of hard and thick tissues compared to other molecular imaging methods because it does not require production of thin tissue slices via microtomes, which greatly simplifies the overall sample preparation procedure and prevents the redistribution of analytes during matrix desorption. Read More