Vimentin contributes to changes in chondrocyte stiffness in osteoarthritis.

Authors:
Prof. Dominik R Haudenschild, Ph.D.
Prof. Dominik R Haudenschild, Ph.D.
University of California Davis Medical Center
Professor
Arthritis Research
Sacramento, CA | United States
Nikolai Steklov
Nikolai Steklov
Shiley Center for Orthopaedic Research and Education at Scripps Clinic
San Diego | United States
Shawn P Grogan
Shawn P Grogan
1 Shiley Center for Orthopaedic Research and Education at Scripps Clinic
United States
Martin K Lotz
Martin K Lotz
The Scripps Research Institute
San Diego | United States

J Orthop Res 2011 Jan;29(1):20-5

Shiley Center for Orthopaedic Research and Education at Scripps Clinic, 11025 North Torrey Pines Road, La Jolla, California 92037, USA.

Actin and tubulin cytoskeletal components are studied extensively in chondrocytes, but less is known about vimentin intermediate filaments. In other cell types, vimentin is a determinant of cell stiffness and disruption of vimentin networks weakens the mechanical integrity of cells. Changes in vimentin organization correlate with osteoarthritis progression, but the functional consequences of these changes remain undetermined in chondrocytes. The objective of this study was to compare the contribution of vimentin to the mechanical stiffness of primary human chondrocytes isolated from normal versus osteoarthritic cartilage. Chondrocytes were embedded in alginate and vimentin networks disrupted with acrylamide. Constructs were imaged while subjected to 20% nominal strain on a confocal microscope stage, and the aspect ratios of approximately 1,900 cells were measured. Cytosolic stiffness was estimated with a finite element model, and live-cell imaging of GFP-vimentin was used to further analyze the nature of vimentin disruption. Vimentin in normal chondrocytes formed an inner cage-like network that was substantially stiffer than the rest of the cytosol and contributed significantly to overall cellular stiffness. Disruption of vimentin reduced stiffness approximately 2.8-fold in normal chondrocytes. In contrast, osteoarthritic chondrocytes were less stiff and less affected by vimentin disruption. This 3D experimental system revealed contributions of vimentin to chondrocyte stiffness previously not apparent, and correlated changes in vimentin-based chondrocyte stiffness with osteoarthritis.

Download full-text PDF

Source
http://dx.doi.org/10.1002/jor.21198DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2976780PMC

Still can't find the full text of the article?

We can help you send a request to the authors directly.
January 2011
14 Reads
22 Citations
2.990 Impact Factor

Publication Analysis

Top Keywords

chondrocyte stiffness
12
disruption vimentin
12
vimentin
12
stiffness osteoarthritis
8
normal chondrocytes
8
vimentin disruption
8
vimentin networks
8
stiffness disruption
8
stiffness
8
chondrocytes
7
stage aspect
4
ratios 1900
4
aspect ratios
4
confocal microscope
4
nominal strain
4
strain confocal
4
1900 cells
4
microscope stage
4
cells measured
4
finite element
4

References

(Supplied by CrossRef)
Induction of chondrogenesis in limb mesenchymal cultures by disruption of the actin cytoskeleton
Zanetti et al.
J Cell Biol 1984
Organisation of the chondrocyte cytoskeleton and its response to changing mechanical conditions in organ culture
Durrant et al.
J Anat 1999
Cytoskeleton of cartilage cells
Benjamin et al.
Microsc Res Tech 1994
The fine structure of chondrocytes
Palfrey et al.
J Anat 1966
Proteomic characterization of human normal articular chondrocytes: a novel tool for the study of osteoarthritis and other rheumatic diseases
Ruiz-Romero et al.
Proteomics 2005
Modulation of tensin and vimentin expression in chick embryo developing cartilage and cultured differentiating chondrocytes
van de Werken et al.
Eur J Biochem 1993
Phenotypic characterization of human chondrocyte cell line C-20/A4: a comparison between monolayer and alginate suspension culture
Finger et al.
Cells Tissues Organs 2004
Cytoskeleton disruption in chondrocytes from a rat osteoarthrosic (OA)-induced model: its potential role in OA pathogenesis
Capin-Gutierrez et al.
Histol Histopathol 2004
Differential proteome analysis of normal and osteoarthritic chondrocytes reveals distortion of vimentin network in osteoarthritis
Lambrecht et al.
Osteoarthritis Cartilage 2008
Increased presence of cells with multiple elongated processes in osteoarthritic femoral head cartilage
Holloway et al.
Osteoarthritis Cartilage 2004

Similar Publications