Radiofrequency field-induced thermal cytotoxicity in cancer cells treated with fluorescent nanoparticles.

Authors:
Evan S Glazer
Evan S Glazer
The University of Texas M. D. Anderson Cancer Center
Steven A Curley
Steven A Curley
The University of Texas MD Anderson Cancer Center
United States

Cancer 2010 Jul;116(13):3285-93

Department of Surgical Oncology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas, USA.

Background: Nonionizing radiation, such as radiofrequency field and near infrared laser, induces thermal cytotoxicity in cancer cells treated with gold nanoparticles. Quantum dots are fluorescent semiconducting nanoparticles that were hypothesized to induce similar injury after radiofrequency field irradiation.

Methods: Gold nanoparticles and 2 types of quantum dot (cadmium-selenide and indium-gallium-phosphide) conjugated to cetuximab (C225), a monoclonal antibody against human epidermal growth factor receptor (EGFR)-1, demonstrated concentration-dependent heating in a radiofrequency field. The authors investigated the effect of radiofrequency field exposure after targeted nanoparticle treatment in a coculture of 2 human cancer cell lines that have differential EGFR-1 expression (a high-expressing pancreatic carcinoma, Panc-1, and a low-expressing breast carcinoma, Cama-1).

Results: Radiofrequency revealed that Panc-1 or Cama-1 cells not containing gold nanoparticles or quantum dots had a viability of > 92%. The viability of Panc-1 cells exposed to the radiofrequency field after treatment with 50 nM Au-C225 was 39.4% +/- 8.3% without injury to bystander Cama-1 cells (viability was 93.7% +/- 1.0%; P approximately .0006). Panc-1 cells treated with targeted cadmium-selenide quantum dots were only 47.5% viable after radiofrequency field exposure (P< .0001 compared with radiofrequency only Panc-1 control cells). Targeted indium-gallium-phosphide quantum dots decreased Panc-1 viability to 58.2% +/- 3.4% after radiofrequency field exposure (P = approximately .0004 compared with Cama-1 and Panc-1 controls).

Conclusions: The authors selectively induced radiofrequency field cytotoxicity in Panc-1 cells without injury to bystander Cama-1 cells using EGFR-1-targeted nanoparticles, and demonstrated an interesting bifunctionality of fluorescent nanoparticles as agents for both cancer cell imaging and treatment.

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http://dx.doi.org/10.1002/cncr.25135DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2928886PMC

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July 2010
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References

(Supplied by CrossRef)
Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destruction
Kam et al.
Proc Natl Acad Sci U S A. 2005
Selective laser photo-thermal therapy of epithelial carcinoma using anti-EGFR antibody conjugated gold nanoparticles
El-Sayed et al.
Cancer Lett. 2006
Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods
Huang et al.
J Am Chem Soc. 2006
Noninvasive radiofrequency field-induced hyperthermic cytotoxicity in human cancer cells using cetuximab-targeted gold nanoparticles
Curley et al.
J Exp Ther Oncol. 2008
Carbon nanotube-enhanced thermal destruction of cancer cells in a noninvasive radiofrequency field
Gannon et al.
Cancer. 2007
Size-dependent joule heating of gold nanoparticles using capacitively coupled radiofrequency fields
Moran et al.
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Theranostic nanomedicine for cancer
Sumer et al.
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Bioconjugation of highly luminescent colloidal CdSe-ZnS quantum dots with an engineered 2-domain recombinant protein
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