Centriole movements in mammalian epithelial cells during cytokinesis.

Authors:
Dr. Károly Szuhai, MD, PhD
Dr. Károly Szuhai, MD, PhD
Leiden University Medical Center
Associate Professor
molecular pathology
Leiden | Netherlands

BMC Cell Biol 2010 May 21;11:34. Epub 2010 May 21.

Cancer Research Laboratory, Faculty of Medicine, University of Iceland, Reykjavik, Iceland.

Background: In cytokinesis, when the cleavage furrow has been formed, the two centrioles in each daughter cell separate. It has been suggested that the centrioles facilitate and regulate cytokinesis to some extent. It has been postulated that termination of cytokinesis (abscission) depends on the migration of a centriole to the intercellular bridge and then back to the cell center. To investigate the involvement of centrioles in cytokinesis, we monitored the movements of centrioles in three mammalian epithelial cell lines, HeLa, MCF 10A, and the p53-deficient mouse mammary tumor cell line KP-7.7, by time-lapse imaging. Centrin1-EGFP and alpha-Tubulin-mCherry were co-expressed in the cells to visualize respectively the centrioles and microtubules.

Results: Here we report that separated centrioles that migrate from the cell pole are very mobile during cytokinesis and their movements can be characterized as 1) along the nuclear envelope, 2) irregular, and 3) along microtubules forming the spindle axis. Centriole movement towards the intercellular bridge was only seen occasionally and was highly cell-line dependent.

Conclusions: These findings show that centrioles are highly mobile during cytokinesis and suggest that the repositioning of a centriole to the intercellular bridge is not essential for controlling abscission. We suggest that centriole movements are microtubule dependent and that abscission is more dependent on other mechanisms than positioning of centrioles.

Download full-text PDF

Source
http://dx.doi.org/10.1186/1471-2121-11-34DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2893098PMC
May 2010
12 Reads
4 Citations
2.341 Impact Factor

Publication Analysis

Top Keywords

intercellular bridge
12
mammalian epithelial
8
centriole intercellular
8
centrioles
8
mobile cytokinesis
8
centriole movements
8
cytokinesis
7
centriole
5
cell
5
co-expressed cells
4
visualize centrioles
4
centrioles microtubulesresults
4
cells visualize
4
separated centrioles
4
pole mobile
4
cytokinesis movements
4
cell pole
4
migrate cell
4
report separated
4
centrioles migrate
4

References

(Supplied by CrossRef)

IA Vorobjev et al.
J Cell Biol 1982

BM Lange et al.
J Cell Biol 1995

A Gromley et al.
J Cell Biol 2003

CT Anderson et al.
Curr Biol 2009

J Azimzadeh et al.
J Cell Sci 2007

PL Alvey et al.
J Cell Sci 1985

M Piehl et al.
Proc Natl Acad Sci 2004

A Khodjakov et al.
Curr Biol 2000

A Khodjakov et al.
J Cell Biol 2001

SC Schaffner et al.
Proc Natl Acad Sci 2006

F Borel et al.
Proc Natl Acad Sci 2002

Similar Publications